Anti-CDC23 Antibody (CAB15116)

Antibody Name:	Anti-CDC23 Antibody
Antibody SKU:	CAB15116
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, 293T, HeLa, Jurkat, HepG2, NIH/3T3, mouse liver, mouse kidney, rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CDC23 (NP_004652.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HSSK WSAE LAFS LPAL PLAE LQPP PPIT EEDA QDMD AYTL AKAY FDVK EYDR AAHF LHGC NSKK AYFL YMYS RYLS GEKK KDDE TVDS LGPL EKGQ VKNE A
Gene ID:	8697
Uniprot:	Q9UJX2
Cellular Location:
Calculated MW:	16kDa/55kDa/68kDa
Observed MW:	69kDa

Synonyms:

CDC23, ANAPC8, APC8, CUT23

Background:

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.

UniProt Protein Function:	CDC23: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC8/CDC23 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 5q31 Cellular Component: nucleoplasm; anaphase-promoting complex; intracellular; cytosol Molecular Function:ubiquitin-protein ligase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; regulation of mitotic metaphase/anaphase transition; mitotic cell cycle spindle assembly checkpoint; regulation of exit from mitosis; mitotic cell cycle; mitotic metaphase plate congression; mitotic metaphase/anaphase transition
NCBI Summary:	The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UJX2
NCBI GenInfo Identifier:	254763423
NCBI Gene ID:	8697
NCBI Accession:	Q9UJX2.3
UniProt Secondary Accession:	Q9UJX2,O75433, Q53FN2, Q9BS73, A8K6E5, B4E3A2, B7WP05 D3DQB7,
UniProt Related Accession:	Q9UJX2
Molecular Weight:	55,882 Da
NCBI Full Name:	Cell division cycle protein 23 homolog
NCBI Synonym Full Names:	cell division cycle 23
NCBI Official Symbol:	CDC23
NCBI Official Synonym Symbols:	APC8; CUT23; ANAPC8
NCBI Protein Information:	cell division cycle protein 23 homolog; cyclosome subunit 8; cell division cycle 23 homolog; anaphase promoting complex subunit 8; anaphase-promoting complex subunit 8
UniProt Protein Name:	Cell division cycle protein 23 homolog
UniProt Synonym Protein Names:	Anaphase-promoting complex subunit 8; APC8; Cyclosome subunit 8
Protein Family:	Anaphase-promoting complex
UniProt Gene Name:	CDC23
UniProt Entry Name:	CDC23_HUMAN

	Western blot analysis of extracts of various cell lines, using CDC23 antibody (CAB15116) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat spleen using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using CDC23 antibody (CAB15116) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
CDC23 Antibody (PACO06335)	Human CDC23 (Cell Division Cycle Protein 23) ELISA Kit (HUES01837)
CDC23 Antibody (PACO08310)	Human Cell division cycle protein 23 homolog (CDC23) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)