Anti-CDC45 Antibody (CAB2047)

Antibody Name:	Anti-CDC45 Antibody
Antibody SKU:	CAB2047
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC45 (NP_003495.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, HeLa, A-549, HL-60, HT-29, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC45 (NP_003495.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFVS DFRK EFYE VVQS QRVL LFVA SDVD ALCA CKIL QALF QCDH VQYT LVPV SGWQ ELET AFLE HKEQ FHYF ILIN CGAN VDLL DILQ PDED TIFF VCDT HRPV NVVN VYND TQIK LLIK QDDD LEVP AYED IFRD EEED EEHS GNDS DGSE PSEK RTRL EEEI VEQT MRRR QRRE WEAR RRDI LFDY EQYE YHGT SSAM VMFE LAWM LSKD LNDM LWWA IVGL TDQW VQDK ITQM KYVT DVGV LQRH VSRH NHRN EDEE NTLS VDCT RI
Gene ID:	8318
Uniprot:	O75419
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	60kDa/65kDa/68kDa
Observed MW:	60kDa

Synonyms:

CDC45, CDC45L, CDC45L2, MGORS7, PORC-PI-1, cdc45

Background:

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	CDC45L: Required for initiation of chromosomal DNA replication. Associated with ORC2. Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. Belongs to the CDC45 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus Molecular Function:protein binding; 3'-5' DNA helicase activity; DNA replication origin binding; chromatin binding; single-stranded DNA binding Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; regulation of chromatin silencing at telomere; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication checkpoint; DNA replication; DNA duplex unwinding; double-strand break repair via break-induced replication; G1/S transition of mitotic cell cycle; pre-replicative complex assembly
NCBI Summary:	The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:	O75419
NCBI GenInfo Identifier:	6685264
NCBI Gene ID:	8318
NCBI Accession:	O75419.1
UniProt Secondary Accession:	O75419,O60856, Q20WK8, Q6UW54, Q9UP68, B4DDB4, B4DDU3 E9PDH7,
UniProt Related Accession:	O75419
Molecular Weight:	566
NCBI Full Name:	Cell division control protein 45 homolog
NCBI Synonym Full Names:	cell division cycle 45
NCBI Official Symbol:	CDC45
NCBI Official Synonym Symbols:	CDC45L; CDC45L2; PORC-PI-1
NCBI Protein Information:	cell division control protein 45 homolog; human CDC45; CDC45-related protein; cell division cycle 45-like 2; cell division cycle 45 homolog; CDC45 cell division cycle 45 homolog
UniProt Protein Name:	Cell division control protein 45 homolog
UniProt Synonym Protein Names:	PORC-PI-1
UniProt Gene Name:	CDC45
UniProt Entry Name:	CDC45_HUMAN

	Western blot analysis of extracts of various cell lines, using cdc45 antibody (CAB2047) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded mouse stomach using cdc45 antibody (CAB2047) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using cdc45 antibody (CAB2047) at dilution of 1:100 (40x lens).

Antibodies

Anti-CDC45 Antibody (CAB16032)

CDC45 Antibody (PACO08322)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)