CDK1 Rabbit Monoclonal Antibody (CAB11420)
- SKU:
- CAB11420
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Product Name: | CDK1 Rabbit Monoclonal Antibody |
| SKU: | CAB11420 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 198-297 of human CDK1 (NP_001777.1). |
| Sequence: | ATKK PLFH GDSE IDQL FRIF RALG TPNN EVWP EVES LQDY KNTF PKWK PGSL ASHV KNLD ENGL DLLS KMLI YDPA KRIS GKMA LNHP YFND LDNQ IKKM |
| Tested Applications: | WB IF/ICC IP ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Synonyms: | CDC2; CDC28A; P34CDC2; CDK1 |
| Positive Sample: | HeLa,MCF7,Jurkat,Mouse spleen |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Mitochondrion, Nucleus, centrosome, cytoskeleton, microtubule organizing center, spindle. |
| Calculated MW: | 34kDa |
| Observed MW: | 34kDa |
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 983 |
| Clone Number: | ARC50607 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using CDK1 Rabbit mAb (CAB11420) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s. |
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