The Phospho-Cdk1/2-T14 Monoclonal Antibody (CABP1001) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Cdk1/2-T14 Monoclonal Antibody
SKU:
CABP1001
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1546
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC2, CDC28A, P34CDC2, Phospho-Cdk1/2-T14
Positive Sample:
HeLa treated with Hydroxyurea, HeLa treated with Nocodazole, NIH/3T3 treated with Hydroxyurea, NIH/3T3 treated with Nocodazole, C6 treated with Hydroxyurea, C6 treated with Nocodazole
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Purification Method
Affinity purification
Gene ID
983 1017
RRID
AB_2863893
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-Cdk1/2-T14 Rabbit mAb (CABP1001) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated with Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated with Nocodazole (100 ng/mL) at 37℃ for 24 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
