The [KO Validated] CDKN1B/p27KIP1 Antibody (CAB16633) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds to the CDKN1B protein, facilitating the detection and analysis of this important cell cycle regulator in various cell types.CDKN1B, also known as p27 or Kip1, acts as a tumor suppressor by inhibiting the activity of cyclin-dependent kinases and regulating cell cycle progression. Dysregulation of CDKN1B has been implicated in various types of cancer, making it a promising target for cancer research.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] CDKN1B/p27KIP1 Antibody
SKU:
CAB16633
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
KIP1, MEN4, CDKN4, MEN1B, P27KIP1, CDKN1B/p27KIP1
Positive Sample:
Mouse lung, Mouse spleen
Cellular Localization:
Cytoplasm, Endosome, Nucleus.
Calculated MW:
22kDa
Observed MW:
27kDa
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).
Purification Method
Affinity purification
Gene ID
1027
RRID
AB_2768831
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDKN1B/p27KIP1 Rabbit pAb (CAB16633) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates using CDKN1B/p27KIP1 Rabbit pAb (CAB16633) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human breast using CDKN1B/p27KIP1 Rabbit pAb (CAB16633) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using CDKN1B/p27KIP1 Rabbit pAb (CAB16633) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
