Anti-CEBPG Antibody (CAB7279)

Antibody Name:	Anti-CEBPG Antibody
Antibody SKU:	CAB7279
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CEBPG (NP_001797.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	A-549, HT-29, Mouse liver, Mouse testis, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CEBPG (NP_001797.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKI SQQN STPG VNGI SVIH TQAH ASGL QQVP QLVP AGPG GGGK AVAP SKQS KKSS PMDR NSDE YRQR RERN NMAV KKSR LKSK QKAQ DTLQ RVNQ LKEE NERL EAKI KLLT KELS VLKD LFLE HAHN LADN VQSI STEN TTAD GDNA GQ
Gene ID:	1054
Uniprot:	P53567
Cellular Location:	Nucleus
Calculated MW:	16kDa
Observed MW:	16kDa

Synonyms:

CEBPG, GPE1BP, IG/EBP-1

Background:

The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	C/EBP-gamma: Transcription factor that binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene. Might change the DNA-binding specificity of other transcription factors and recruit them to unusual DNA sites. Belongs to the bZIP family. C/EBP subfamily.
UniProt Protein Details:	Protein type:DNA-binding; Motility/polarity/chemotaxis; Transcription factor Chromosomal Location of Human Ortholog: 19q13.11 Cellular Component: nucleoplasm; nucleus Molecular Function:DNA binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transcription factor binding Biological Process: B cell differentiation; enucleate erythrocyte differentiation; immune response; liver development; mRNA metabolic process; natural killer cell mediated cytotoxicity; negative regulation of transcription factor activity; positive regulation of DNA binding; positive regulation of DNA repair; positive regulation of interferon-gamma biosynthetic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
NCBI Summary:	The C/EBP family of transcription factors regulates viral and cellular CCAAT/enhancer element-mediated transcription. C/EBP proteins contain the bZIP region, which is characterized by two motifs in the C-terminal half of the protein: a basic region involved in DNA binding and a leucine zipper motif involved in dimerization. The C/EBP family consist of several related proteins, C/EBP alpha, C/EBP beta, C/EBP gamma, and C/EBP delta, that form homodimers and that form heterodimers with each other. CCAAT/enhancer binding protein gamma may cooperate with Fos to bind PRE-I enhancer elements. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2011]
UniProt Code:	P53567
NCBI GenInfo Identifier:	1705750
NCBI Gene ID:	1054
NCBI Accession:	P53567.1
UniProt Secondary Accession:	P53567,Q5U052, B2R946,
UniProt Related Accession:	P53567
Molecular Weight:	16,408 Da
NCBI Full Name:	CCAAT/enhancer-binding protein gamma
NCBI Synonym Full Names:	CCAAT/enhancer binding protein gamma
NCBI Official Symbol:	CEBPG
NCBI Official Synonym Symbols:	GPE1BP; IG/EBP-1
NCBI Protein Information:	CCAAT/enhancer-binding protein gamma
UniProt Protein Name:	CCAAT/enhancer-binding protein gamma
Protein Family:	CCAAT/enhancer-binding protein
UniProt Gene Name:	CEBPG
UniProt Entry Name:	CEBPG_HUMAN

	Western blot analysis of extracts of various cell lines, using CEBPG antibody (CAB7279) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using CEBPG antibody (CAB7279) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using CEBPG antibody (CAB7279) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using CEBPG antibody (CAB7279) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using CEBPG antibody (CAB7279) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
CEBPG Antibody (PACO02334)	Human CEBPgamma (CCAAT/Enhancer Binding Protein Gamma) ELISA Kit (HUES01834)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)