The CFAP53 Antibody (CAB16607) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly reactive with human samples, making it ideal for use in Western blot applications. By binding to the CFAP53 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it a crucial asset for studies in cell biology and cilia-related diseases.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CFAP53 Antibody
SKU:
CAB16607
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HTX6, CCDC11, CFAP53
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Cell Projection, Cilium.
Calculated MW:
62kDa
Observed MW:
62kDa
This gene belongs to the CFAP53 family. It was found to be differentially expressed by the ciliated cells of frog epidermis and in skin fibroblasts from human. Mutations in this gene are associated with visceral heterotaxy-6, which implicates this gene in determination of left-right asymmetric patterning.
Purification Method
Affinity purification
Gene ID
220136
RRID
AB_2768867
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CFAP53 Rabbit pAb (CAB16607) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of HeLa cells using CFAP53 Rabbit pAb (CAB16607) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
