The CFHR3 Antibody (CAB7775) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits strong reactivity towards human samples and is validated for use in various applications including Western blotting. By targeting the CFHR3 protein, this antibody enables precise detection and analysis in different cell types, making it an ideal tool for investigations in immunology and related fields.CFHR3, a complement factor H-related protein, plays a vital role in regulating the complement cascade and immune response. Dysregulation of CFHR3 is associated with various autoimmune diseases, inflammatory conditions, and renal disorders.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CFHR3 Antibody
SKU:
CAB7775
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FHR3, HLF4, CFHL3, FHR-3, DOWN16, CFHR3
Positive Sample:
Rat liver
Cellular Localization:
Secreted.
Calculated MW:
37kDa
Observed MW:
37kDa
The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-related macular degeneration, and with an increased risk of atypical hemolytic-uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10878
RRID
AB_2768870
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat liver, using CFHR3 Rabbit pAb (CAB7775) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse liver using CFHR3 Rabbit pAb (CAB7775) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
