The cGAS Antibody (CAB8335) is a high-quality antibody developed for reliable detection and analysis of target proteins. Produced in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. It binds to cGAS, facilitating its detection and analysis in various cell types, making it an essential reagent for investigations in immunology and infectious disease research.cGAS, also known as cyclic GMP-AMP synthase, functions as a cytosolic DNA sensor, triggering signaling pathways that lead to the production of type I interferons and pro-inflammatory cytokines.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
cGAS Antibody
SKU:
CAB8335
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MB21D1, h-cGAS, C6orf150, cGAS
Positive Sample:
HeLa, HT-29, THP-1
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
59kDa
Observed MW:
62kDa
Enables several functions, including 2',3'-cyclic GMP-AMP synthase activity; chromatin binding activity; and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in several processes, including cellular response to exogenous dsRNA; positive regulation of intracellular signal transduction; and regulation of defense response. Located in several cellular components, including cytosol; nucleus; and site of double-strand break.
Purification Method
Affinity purification
Gene ID
115004
RRID
AB_2770305
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using cGAS Rabbit pAb (CAB8335) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from HT-29 cells using cGAS Rabbit pAb (CAB8335) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using cGAS Rabbit pAb (CAB8335) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using cGAS Rabbit pAb (CAB8335) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of PC-12 cells using cGAS Rabbit pAb (CAB8335) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using cGAS Rabbit pAb (CAB8335) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
