Anti-CHD4 Antibody (CAB11574)

Antibody Name:	Anti-CHD4 Antibody
Antibody SKU:	CAB11574
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1690 of human CHD4 (NP_001264.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, 293T, HT-1080

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1690 of human CHD4 (NP_001264.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ELAE VEEN KKMS QPGS PSPK TPTP STPG DTQP NTPA PVPP AEDG IKIE ENSL KEEE SIEG EKEV KSTA PETA IECT QAPA PASE DEKV VVEP PEGE EKVE KAEV KERT EEPM ETEP KGAA DVEK VEEK SAID LTPI VVED KEEK KEEE EKKE VMLQ NGET PKDL NDEK QKK
Gene ID:	1108
Uniprot:	Q14839
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	218kDa/220kDa
Observed MW:	280kDa

Synonyms:

CHD4, CHD-4, Mi-2b, Mi2-BETA, SIHIWES

Background:

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
NCBI Summary:	The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
UniProt Code:	Q14839
NCBI GenInfo Identifier:	311033360
NCBI Gene ID:	1108
NCBI Accession:	Q14839.2
UniProt Secondary Accession:	Q14839,Q8IXZ5,
UniProt Related Accession:	Q14839
Molecular Weight:	278 kDa
NCBI Full Name:	Chromodomain-helicase-DNA-binding protein 4
NCBI Synonym Full Names:	chromodomain helicase DNA binding protein 4
NCBI Official Symbol:	CHD4
NCBI Official Synonym Symbols:	CHD-4; Mi-2b; SIHIWES; Mi2-BETA
NCBI Protein Information:	chromodomain-helicase-DNA-binding protein 4
UniProt Protein Name:	Chromodomain-helicase-DNA-binding protein 4
UniProt Synonym Protein Names:	ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta
Protein Family:	Chromodomain-helicase-DNA-binding protein
UniProt Gene Name:	CHD4

	Western blot analysis of extracts of various cell lines, using CHD4 antibody (CAB11574) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Rat liver using CHD4 antibody (CAB11574) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human oophoroma using CHD4 antibody (CAB11574) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse liver using CHD4 antibody (CAB11574) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using CHD4 antibody (CAB11574) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

CHD4 Antibody (PACO03378)

CHD4 Antibody (PACO08399)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)