The Chicken APOA1 (Apolipoprotein A1) ELISA Kit, offered by Assay Genie, is a cutting-edge solution for precise measurement of APOA1 levels in chicken serum, plasma, and cell culture supernatants. This advanced kit delivers exceptional sensitivity and specificity, ensuring the accuracy and reproducibility of experimental data. APOA1, a key component of high-density lipoprotein (HDL) particles, plays a vital role in lipid metabolism and cardiovascular health.
As a crucial biomarker, variations in APOA1 levels are associated with various metabolic disorders, cardiovascular diseases, and inflammation. The utilization of the Chicken APOA1 ELISA Kit provides researchers with a reliable tool for exploring the role of APOA1 in disease pathogenesis, drug development, and therapeutic interventions. Discover new insights and drive groundbreaking research with the Chicken APOA1 ELISA Kit from Assay Genie.
Product Name:
Chicken ApoA1 (Apolipoprotein A1) ELISA Kit
SKU:
AEFI00026
Reactivity:
Chicken
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 ng/mL
Range:
15.6-1000 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
98-104%
85-103%
86-103%
EDTA Plasma (n = 5)
84-100%
86-101%
85-96%
Heparin Plasma (n = 5)
94-105%
81-99%
88-95%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-102
94
EDTA Plasma (n = 5)
85-98
92
Heparin Plasma (n = 5)
85-105
91
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
