Chicken HSP90AA1 (Heat shock protein HSP 90-alpha) ELISA Kit
The Chicken HSP90AA1 (Heat Shock Protein Hsp 90 Alpha) ELISA Kit is a specialized assay designed for precise and accurate measurement of chicken HSP90AA1 levels in various sample types, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, offering dependable and consistent results, making it a valuable tool for a wide array of research applications. HSP90AA1 is a key protein belonging to the heat shock protein family, playing a critical role in cell stress response and protein folding. It is involved in diverse cellular processes, including cell signaling, protein degradation, and immune responses, making it a significant target for studying various physiological and pathological conditions.
Understanding the expression levels of HSP90AA1 can provide insights into cellular stress, protein homeostasis, and disease progression. By accurately quantifying HSP90AA1 levels, researchers can unravel its role in conditions such as cancer, neurodegenerative disorders, and inflammatory diseases. This ELISA kit is an indispensable tool for investigating the functions of HSP90AA1 and exploring its potential as a therapeutic target in various diseases.
Product Name:
Chicken HSP90AA1 (Heat shock protein HSP 90-alpha) ELISA Kit
SKU:
AEFI00022
Reactivity:
Chicken
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
1.875 ng/mL
Range:
3.125-200 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-105%
87-96%
87-95%
EDTA Plasma (n = 5)
82-98%
87-100%
83-101%
Heparin Plasma (n = 5)
84-99%
83-92%
81-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-105
96
EDTA Plasma (n = 5)
85-105
95
Heparin Plasma (n = 5)
85-100
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
