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Chicken SOD1 (Superoxide dismutase [Cu-Zn]) ELISA Kit (CHFI00106)

Datasheet MSDS
SKU CHFI00106
Product Type ELISA Kit
Size 48T/96T
Reactivity Chicken
Detection Method Sandwich ELISA, Double Antibody
Sensitivity 0.469 ng/mL
Range 0.78-50 ng/mL (20000IU/mg)
€649

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Description

system_update_altDatasheetsystem_update_altMSDS

Chicken SOD1 (Superoxide dismutase [Cu-Zn]) ELISA Kit

The Chicken SOD1 (Superoxide Dismutase Cu-Zn) ELISA Kit is specifically designed for the detection of SOD1 levels in chicken samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in serum, plasma, and cell culture supernatants. SOD1 is an important enzyme that plays a crucial role in protecting cells from oxidative stress by breaking down superoxide radicals. This enzyme is essential for maintaining cellular health and is implicated in various diseases, including neurodegenerative disorders and inflammatory conditions.

By accurately measuring SOD1 levels in chicken samples, researchers can gain valuable insights into oxidative stress levels and potential therapeutic targets. This ELISA kit provides a reliable tool for studying the role of SOD1 in chicken health and disease, making it an essential component of research in avian biology and veterinary medicine.

Product Name:Chicken SOD1 (Superoxide dismutase [Cu-Zn]) ELISA Kit
SKU:CHFI00106
Reactivity:Chicken
Assay Type:Sandwich ELISA, Double Antibody
Sensitivity:0.469 ng/mL
Range:0.78-50 ng/mL (20000IU/mg)
Sample Type:Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:2-8°C for 12 months.
Linearity:
Sample1:21:41:8
Serum (n = 5)89-105%82-100%83-99%
EDTA Plasma (n = 5)89-102%82-94%81-97%
Heparin Plasma (n = 5)85-102%84-97%89-96%
Recovery:
SampleRecovery Range (%)Average (%)
Serum (n = 5)87-10393
EDTA Plasma (n = 5)86-9891
Heparin Plasma (n = 5)86-10194
Assay Procedure

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample TypeProtocol
SerumAllow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
PlasmaCollect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue HomogenateHomogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture SupernatantCentrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell LysateLyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample TypesFor more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
ComponentQuantityStorage
48T96T
ELISA Microplate (Dismountable)8×68×12Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard1 vial2 vialPlace the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)60 ul120 ul2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)60 ul120 ul2-8°C (Avoid direct light)
TMB Substrate5 ml10 ml2-8°C (Avoid direct light)
Sample Dilution Buffer10 ml20 ml2-8°C
Antibody Dilution Buffer5 ml10 ml2-8°C
SABC Dilution Buffer5 ml10 ml2-8°C
Stop Solution5 ml10 ml2-8°C
Wash Buffer(25X)15 ml30 ml2-8°C
Plate Sealer3 pieces5 pieces-
Technical Manual1 copy1 copy-
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