Description
system_update_altDatasheet
| Product Name: | Chloroplast Isolation Kit |
| Product Code: | ARMA00350 |
| Product Size: | 30 x 1 g Plant Tissue Extractions |
| Detection Principle: | Density-Cushion Differential Centrifugation |
| Storage: | 4°C (unopened kit) |
The Chloroplast Isolation Kit provides all buffers and reagents required for rapid isolation of intact, functional chloroplasts from plant tissue. Chloroplasts are isolated using density-cushion differential centrifugation, utilizing an Optiprep density medium to separate intact chloroplasts from broken chloroplasts and other cellular debris.
| Tissue Sample: | Approximately 1 g |
| Pre-treatment: | Store in dark and cold for at least 4 hours (12-24 hours preferable) |
| Homogenization: | 4-6 up and down strokes with loose pestle or bead beater at 25-0-25 Hz for 3-5 seconds |
| Centrifugation Steps: | 200 × g (3 min), 1500 × g (5 min), 3000 × g (10 min with brake disabled) |
| Temperature: | 4°C throughout procedure |
| Lighting: | Subdued lighting where possible |
| Final Storage: | 200 µl in Chloroplast Storage Buffer, on ice in dark |
| Kit Storage (Unopened): | 4°C |
| All Buffers & Apparatus: | Keep on ice before and during isolation |
| Isolated Chloroplasts (Fresh): | 4-6 hours on ice in dark (loses up to 40% activity overnight) |
| Frozen Storage (-80°C): | Maximum 1-2 months; 80-90% function preserved at 2 weeks, 40-50% at 1-2 months |
| Cryopreservation: | Add 5% DMSO and 10% glycerol; freeze slowly using controlled-rate freezing container |
| Thawing: | Rapid thaw at 25-30°C water bath until sliver of ice remains |
