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Anti-CHMP1B Antibody (CAB8239)

SKU:
CAB8239
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 2 Anti-CHMP1B Antibody CAB8239
  • Cell Cycle Antibodies 2 Anti-CHMP1B Antibody CAB8239
  • Cell Cycle Antibodies 2 Anti-CHMP1B Antibody CAB8239
  • Cell Cycle Antibodies 2 Anti-CHMP1B Antibody CAB8239
€139 - €419
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Description

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Antibody Name:CHMP1B Rabbit Polyclonal Antibody
Antibody SKU:CAB8239
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human CHMP1B (NP_065145.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:NCI-H460, MCF7, SKOV3, U-251MG, Mouse liver, Mouse spleen, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human CHMP1B (NP_065145.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSNM EKHL FNLK FAAK ELSR SAKK CDKE EKAE KAKI KKAI QKGN MEVA RIHA ENAI RQKN QAVN FLRM SARV DAVA ARVQ TAVT MGKV TKSM AGVV KSMD ATLK TMNL EKIS ALMD KFEH QFET LDVQ TQQM EDTM SSTT TLTT PQNQ VDML LQEM ADEA GLDL NMEL PQGQ TGSV GTSV ASAE QDEL SQRL ARLR DQV
Gene ID:57132
Uniprot:Q7LBR1
Cellular Location:Cytoplasm, Endosome, Late endosome membrane, Peripheral membrane protein, cytosol
Calculated MW:22kDa
Observed MW:30kDa
Synonyms:CHMP1B, C10orf2, C18-ORF2, C18orf2, CHMP1.5, Vps46-2, Vps46B, hVps46-2
Background:
UniProt Protein Function:Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells. Involved in HIV-1 p6- and p9-dependent virus release.
NCBI Summary:CHMP1B belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]
UniProt Code:Q7LBR1
NCBI GenInfo Identifier:31542306
NCBI Gene ID:57132
NCBI Accession:NP_065145
UniProt Secondary Accession:Q7LBR1,Q96E89, Q9HD41,
UniProt Related Accession:Q7LBR1
Molecular Weight:24.7 kDa (223aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
NCBI Full Name:charged multivesicular body protein 1b
NCBI Synonym Full Names:charged multivesicular body protein 1B
NCBI Official Symbol:CHMP1B  
NCBI Official Synonym Symbols:Vps46B; C10orf2; C18orf2; CHMP1.5; Vps46-2; C18-ORF2; hVps46-2  
NCBI Protein Information:charged multivesicular body protein 1b
UniProt Protein Name:Charged multivesicular body protein 1b
UniProt Synonym Protein Names:CHMP1.5; Chromatin-modifying protein 1b; CHMP1b; Vacuolar protein sorting-associated protein 46-2; Vps46-2; hVps46-2
Protein Family:Charged multivesicular body protein
UniProt Gene Name:CHMP1B  
Anti-CHMP1B Polyclonal Antibody (CAB8239)Western blot analysis of extracts of various cell lines, using CHMP1B antibody (CAB8239) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CHMP1B Polyclonal Antibody (CAB8239)Immunohistochemistry of paraffin-embedded mouse kidney using CHMP1B antibody (CAB8239) at dilution of 1:100 (40x lens).
Anti-CHMP1B Polyclonal Antibody (CAB8239)Immunohistochemistry of paraffin-embedded rat brain using CHMP1B antibody (CAB8239) at dilution of 1:100 (40x lens).
Anti-CHMP1B Polyclonal Antibody (CAB8239)Immunohistochemistry of paraffin-embedded human liver damage using CHMP1B antibody (CAB8239) at dilution of 1:100 (40x lens).

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