Choline Assay Kit (BA0088)
Choline Assay Kit - InformationAssay Genie's choline assay provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to ine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
ApplicationsFor quantitative determination of choline and evaluation of drug effects on choline metabolism.
Choline Assay Kit - Key Features
- Use 20 samples. Linear detection range: colorimetric assay 1 to 100 , fluorimetric assay 0.2 to 10 choline.
Choline Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL Enzyme Mix: Dried Dye Reagent: 120 uL Standard: 400 uL 2 mM Choline|
|Kit Requires||Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||OD, FL: 1, 0.2|
|Samples||Serum, plasma, urine, saliva, milk, tissue, cells etc|
|Protocol Length||30 min|
|Storage||Store all components at -20°C|
|Shelf Life||6 months|
More DetailsCHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety.