Choline Assay Kit (BA0088)

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ELISA Kit Technical ManualMSDS

Choline Assay Kit - Information

Assay Genie's choline assay provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to ž†ine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.


For quantitative determination of choline and evaluation of drug effects on choline metabolism.

Choline Assay Kit - Key Features

  • Use 20 samples. Linear detection range: colorimetric assay 1 to 100 , fluorimetric assay 0.2 to 10 choline.

Choline Assay Kit - Data Sheet

Kit IncludesAssay Buffer: 10 mL Enzyme Mix: Dried Dye Reagent: 120 uL Standard: 400 uL 2 mM Choline
Kit RequiresPipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader
Method of DetectionOD570nm, or FL530/585nm
Detection LimitOD, FL: 1, 0.2
SamplesSerum, plasma, urine, saliva, milk, tissue, cells etc
Protocol Length30 min
Size100 tests
StorageStore all components at -20°C
Shelf Life6 months

More Details

CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety.
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