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Choline Assay Kit - Information
Assay Genie's choline assay provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
For quantitative determination of choline and evaluation of drug effects on choline metabolism.
Choline Assay Kit - Key Features
- Use 20 samples. Linear detection range: colorimetric assay 1 to 100 , fluorimetric assay 0.2 to 10 choline.
Choline Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL Enzyme Mix: Dried Dye Reagent: 120 uL Standard: 400 uL 2 mM Choline|
|Kit Requires||Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||OD, FL: 1, 0.2|
|Samples||Serum, plasma, urine, saliva, milk, tissue, cells etc|
|Protocol Length||30 min|
|Storage||Store all components at -20°C|
|Shelf Life||6 months|
CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety.