Acetylcholine Assay Kit (BA0069)
Acetylcholine Assay Kit - InformationAssay Genie's acetylcholine assay provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.
ApplicationsFor quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism.
Acetylcholine Assay Kit - Key Features
- Use 20 samples. Linear detection range: colorimetric assay 10 to 200 , fluorimetric assay 0.4 to 10 acetylcholine.
Acetylcholine Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL ACHE Enzyme: 120 mL Enzyme Mix: Dried Dye Reagent: 120 mL Standard: 400 mL 2 mM acetylcholine|
|Kit Requires||Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader.|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||OD, FL: 10, 0.4|
|Samples||Serum, plasma, urine, saliva, milk, tissue, and cell culture etc|
|Protocol Length||30 min|
|Storage||Store all components at -20°C.|
|Shelf Life||6 months|
More DetailsACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood.