Acetylcholine Assay Kit - Information
Assay Genie's acetylcholine assay provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2
. The resulting H2
reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.
For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism.
Acetylcholine Assay Kit - Key Features
- Use 20 samples. Linear detection range: colorimetric assay 10 to 200 , fluorimetric assay 0.4 to 10 acetylcholine.
Acetylcholine Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL ACHE Enzyme: 120 mL Enzyme Mix: Dried Dye Reagent: 120 mL Standard: 400 mL 2 mM acetylcholine|
|Kit Requires||Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flatbottom uncoated 96-well plates, fluorescence plate reader.|
|Method of Detection||OD570nm, or FL530/585nm|
|Detection Limit||OD, FL: 10, 0.4 |
|Samples||Serum, plasma, urine, saliva, milk, tissue, and cell culture etc|
|Protocol Length||30 min|
|Storage||Store all components at -20°C.|
|Shelf Life||6 months|
is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory and mood.