The CHRD Antibody (CAB17571) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high specificity and sensitivity for detecting CHRDL in human samples, making it ideal for use in Western blot analyses. By specifically binding to CHRDL, researchers can investigate its functions in various cell types and gain insights into its potential roles in skeletal development and disease.CHRDL, also known as chordin-like protein, is a key player in the regulation of bone morphogenetic proteins (BMPs), which are essential for embryonic development and bone formation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
CHRD Antibody
SKU:
CAB17571
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CHRD
Positive Sample:
Mouse liver, Rat liver
Cellular Localization:
Extracellular Space.
Calculated MW:
102kDa
Observed MW:
102kDa
This gene encodes a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The encoded protein may also have roles in organogenesis and during adulthood. It has been suggested that this gene could be a candidate gene for Cornelia de Lange syndrome. Reduced expression of this gene results in enhanced bone regeneration. Alternative splicing results in multiple transcript variants. Other alternative splice variants have been described but their full length sequence has not been determined.
Purification Method
Affinity purification
Gene ID
8646
RRID
AB_2768905
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CHRD Rabbit pAb (CAB17571) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
