The CKAP4 Antibody (CAB7777) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables RNA binding activity. Located in several cellular components, including lipid droplet; nuclear speck; and rough endoplasmic reticulum.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CKAP4 Antibody
SKU:
CAB7777
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p63, CLIMP63, CLIMP-63, ERGIC-63, CKAP4
Positive Sample:
293T, HepG2, MCF7, NCI-H460, Mouse testis
Cellular Localization:
Cell Membrane, Cytoplasm, Endoplasmic Reticulum Membrane, Single-Pass Type Ii Membrane Protein, Cytoskeleton, Perinuclear Region.
Calculated MW:
66kDa
Observed MW:
66kDa
Enables RNA binding activity. Located in several cellular components, including lipid droplet; nuclear speck; and rough endoplasmic reticulum.
Purification Method
Affinity purification
Gene ID
10970
RRID
AB_2768932
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CKAP4 Rabbit pAb (CAB7777) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Confocal immunofluorescence analysis of U2OS cells using CKAP4 Rabbit pAb (CAB7777) at dilution of 1:100. Blue: DAPI for nuclear staining.
