Anti-CKAP5 Antibody (CAB8532)

Antibody Name:	Anti-CKAP5 Antibody
Antibody SKU:	CAB8532
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat, HepG2, HT-29, Mouse brain, Mouse lung, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1763-2032 of human CKAP5 (NP_001008938.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLHT LCKL KGPK ILDH LTMI DNKN ESEL EAHL CRMM KHSM DQTG SKSD KETE KGAS RIDE KSSK AKVN DFLA EIFK KIGS KENT KEGL AELY EYKK KYSD ADIE PFLK NSSQ FFQS YVER GLRV IEME REGK GRIS TSTG ISPQ MEVT CVPT PTST VSSI GNTN GEEV GPSV YLER LKIL RQRC GLDN TKQD DRPP LTSL LSKP AVPT VASS TDML HSKL SQLR ESRE QHQH SDLD SNQT HSSG TVTS SSST ANID DLKK RLER IKSS RK
Gene ID:	9793
Uniprot:	Q14008
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Calculated MW:	218kDa/225kDa/226kDa
Observed MW:	250kDa

Synonyms:

CKAP5, CHTOG, MSPS, TOG, TOGp, ch-TOG

Background:

This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	CKAP5: Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. Belongs to the TOG/XMAP215 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: microtubule cytoskeleton; spindle pole; centrosome; protein complex; membrane; cytosol; gamma-tubulin complex Molecular Function:protein binding Biological Process: mitosis; centrosome organization and biogenesis; cell division; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; spindle organization and biogenesis; RNA transport; establishment and/or maintenance of microtubule cytoskeleton polarity
NCBI Summary:	This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
UniProt Code:	Q14008
NCBI GenInfo Identifier:	57164942
NCBI Gene ID:	9793
NCBI Accession:	NP_001008938.1
UniProt Secondary Accession:	Q14008,Q05D70, Q0VAX7, Q0VAX8, Q14668, Q2TA89, Q6NSH4
UniProt Related Accession:	Q14008
Molecular Weight:	226,258 Da
NCBI Full Name:	cytoskeleton-associated protein 5 isoform a
NCBI Synonym Full Names:	cytoskeleton associated protein 5
NCBI Official Symbol:	CKAP5
NCBI Official Synonym Symbols:	TOG; MSPS; TOGp; CHTOG; ch-TOG
NCBI Protein Information:	cytoskeleton-associated protein 5; colonic and hepatic tumor overexpressed gene protein; colonic and hepatic tumor over-expressed gene protein
UniProt Protein Name:	Cytoskeleton-associated protein 5
UniProt Synonym Protein Names:	Colonic and hepatic tumor overexpressed gene protein; Ch-TOG
Protein Family:	Cytoskeleton-associated protein
UniProt Gene Name:	CKAP5
UniProt Entry Name:	CKAP5_HUMAN

	Western blot analysis of extracts of various cell lines, using CKAP5 antibody (CAB8532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using CKAP5 antibody (CAB8532) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Rat H9C2 cells using CKAP5 antibody (CAB8532) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Mouse L929 cells using CKAP5 antibody (CAB8532) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug CKAP5 antibody (CAB8532). Western blot was performed from the immunoprecipitate using CKAP5 antibody (CAB8532) at a dilition of 1:1000.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)