Anti-CLASP1 Antibody (CAB7081)

Antibody Name:	Anti-CLASP1 Antibody
Antibody SKU:	CAB7081
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1389-1538 of human CLASP1 (NP_056097.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, MCF7, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1389-1538 of human CLASP1 (NP_056097.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IMKT LEAH KDSH KEVV RAAE EAAS TLAS SIHP EQCI KVLC PIIQ TADY PINL AAIK MQTK VVER IAKE SLLQ LLVD IIPG LLQG YDNT ESSV RKAS VFCL VAIY SVIG EDLK PHLA QLTG SKMK LLNL YIKR AQTT NSNS SSSS DVST HS
Gene ID:	23332
Uniprot:	Q7Z460
Cellular Location:	Chromosome, Cytoplasm, Golgi apparatus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle, trans-Golgi network
Calculated MW:	162kDa/163kDa/164kDa/169kDa
Observed MW:	169kDa

Synonyms:	CLASP1, MAST1
Background:

UniProt Protein Function:	CLASP1: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Belongs to the CLASP family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q14.2-q14.3 Cellular Component: kinetochore; kinetochore microtubule; Golgi apparatus; centrosome; membrane; cytoplasmic microtubule; spindle microtubule; cortical microtubule cytoskeleton; cell cortex; cytosol Molecular Function:kinetochore binding; microtubule plus-end binding; protein binding; microtubule binding Biological Process: axon guidance; microtubule organizing center organization and biogenesis; negative regulation of microtubule depolymerization; organelle organization and biogenesis; negative regulation of microtubule polymerization or depolymerization; establishment and/or maintenance of cell polarity; establishment of spindle orientation; microtubule cytoskeleton organization and biogenesis; microtubule nucleation; cell division; mitotic cell cycle; G2/M transition of mitotic cell cycle; exit from mitosis; microtubule bundle formation
NCBI Summary:	CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q7Z460
NCBI GenInfo Identifier:	74723323
NCBI Gene ID:	23332
NCBI Accession:	Q7Z460.1
UniProt Secondary Accession:	Q7Z460,O75118, Q2KHQ9, Q5H9P0, Q8N5B8, Q9BQT5, A2RU21 B7ZLX3, F5GWS0,
UniProt Related Accession:	Q7Z460
Molecular Weight:	1538
NCBI Full Name:	CLIP-associating protein 1
NCBI Synonym Full Names:	cytoplasmic linker associated protein 1
NCBI Official Symbol:	CLASP1
NCBI Official Synonym Symbols:	MAST1
NCBI Protein Information:	CLIP-associating protein 1; multiple asters 1; protein Orbit homolog 1; multiple asters homolog 1; cytoplasmic linker-associated protein 1
UniProt Protein Name:	CLIP-associating protein 1
UniProt Synonym Protein Names:	Cytoplasmic linker-associated protein 1; Multiple asters homolog 1; Protein Orbit homolog 1; hOrbit1
Protein Family:	CLIP-associating protein
UniProt Gene Name:	CLASP1
UniProt Entry Name:	CLAP1_HUMAN

	Western blot analysis of extracts of various cell lines, using CLASP1 antibody (CAB7081) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse ileum using CLASP1 antibody (CAB7081) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using CLASP1 antibody (CAB7081). Blue: DAPI for nuclear staining.

Antibodies

CLASP1 Antibody (PACO02766)

CLASP1 Antibody (PACO08450)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)