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Clathrin heavy chain Rabbit Monoclonal Antibody (CAB4943)

Datasheet MSDS
SKU CAB4943
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms Hc, CHC, CHC17, MRD56, CLH-17, CLTCL2, Clathrin heavy chain
Research Use Autophagy
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

Clathrin heavy chain Monoclonal Antibody (CAB4943)

The Clathrin heavy chain Monoclonal Antibody (CAB4943) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from rabbit hosts, exhibits high specificity and reactivity towards the clathrin heavy chain protein in various species, including human samples.Clathrin is a key protein involved in the formation of coated vesicles, which play a critical role in the internalization and transport of molecules within cells. By targeting the clathrin heavy chain, this antibody enables the precise detection and visualization of clathrin-coated structures in cells, making it an essential reagent for studies in cell biology, neuroscience, and drug delivery research.

This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Clathrin heavy chain Monoclonal Antibody
SKU:CAB4943
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC1228
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:SFAQ FKME GNAE ESTL FCFA VRGQ AGGK LHII EVGT PPTG NQPF PKKA VDVF FPPE AQND FPVA MQIS EKHD VVFL ITKY GYIH LYDL ETGT CIYM NRIS G
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:
WB1:1000 - 1:6000
IHC-P1:200 - 1:2000
IF/ICC1:100 - 1:400
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:Hc, CHC, CHC17, MRD56, CLH-17, CLTCL2, Clathrin heavy chain
Positive Sample:NIH/3T3, Jurkat, C6, Mouse kidney, Rat kidney
Cellular Localization:Cytoplasmic Side, Cytoplasmic Vesicle Membrane, Melanosome, Membrane, Peripheral Membrane Protein, Coated Pit.
Calculated MW:191kDa
Observed MW:192kDa

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.

Purification MethodAffinity purification
Gene ID1213 8218
RRIDAB_2863395
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using Clathrin heavy chain Rabbit mAb (CAB4943) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Clathrin heavy chain Rabbit mAb (CAB4943) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Clathrin heavy chain Rabbit mAb (CAB4943) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageConfocal imaging of HeLa cells using Clathrin heavy chain Rabbit mAb (CAB4943, dilution 1:100) (Red). The cells were counterstained with α-Tubulin Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
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