Clathrin heavy chain Monoclonal Antibody (CAB4943)
The Clathrin heavy chain Monoclonal Antibody (CAB4943) is a high-quality antibody developed for reliable detection and analysis of target proteins. Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. RRID AB_2863395 Gene ID 1213 8218 Swiss Prot Synonym Hc; CHC; CHC17; MRD56; CLH-17; CLTCL2; Clathrin heavy chain
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Clathrin heavy chain Monoclonal Antibody
SKU:
CAB4943
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1228
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB
1:1000 - 1:6000
IHC-P
1:200 - 1:2000
IF
/
ICC
1:100 - 1:400
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Hc, CHC, CHC17, MRD56, CLH-17, CLTCL2, Clathrin heavy chain
Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. RRID AB_2863395 Gene ID 1213 8218 Swiss Prot Synonym Hc; CHC; CHC17; MRD56; CLH-17; CLTCL2; Clathrin heavy chain
Purification Method:
Affinity purification
Gene ID:
1213 8218
RRID:
AB_2863395
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Clathrin heavy chain Rabbit mAb (CAB4943) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Clathrin heavy chain Rabbit mAb (CAB4943) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Clathrin heavy chain Rabbit mAb (CAB4943) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of HeLa cells using Clathrin heavy chain Rabbit mAb (CAB4943, dilution 1:100) (Red). The cells were counterstained with α-Tubulin Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
