Anti-CMAS Antibody (CAB13752)

Antibody Name:	Anti-CMAS Antibody
Antibody SKU:	CAB13752
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human CMAS (NP_061156.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HT-29, 293T, HepG2, DU145, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-263 of human CMAS (NP_061156.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDSV EKGA ATSV SNPR GRPS RGRP PKLQ RNSR GGQG RGVE KPPH LAAL ILAR GGSK GIPL KNIK HLAG VPLI GWVL RAAL DSGA FQSV WVST DHDE IENV AKQF GAQV HRRS SEVS KDSS TSLD AIIE FLNY HNEV DIVG NIQA TSPC LHPT DLQK VAEM IREE GYDS VFSV VRRH QFRW SEIQ KGVR EVTE PLNL NPAK RPRR QDWD GELY ENGS FYFA KRHL IEMG YLQG GKMA YYEM RAEH SVDI DVDI DWPI AEQR VLR
Gene ID:	55907
Uniprot:	Q8NFW8
Cellular Location:	Nucleus
Calculated MW:	29kDa/48kDa
Observed MW:	48kDa

Synonyms:	CMAS, CSS
Background:	This gene encodes an enzyme that converts N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc). This process is important in the formation of sialylated glycoprotein and glycolipids. This modification plays a role in cell-cell communications and immune responses. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CMAS: Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Belongs to the CMP-NeuNAc synthase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.7.7.43; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase Chromosomal Location of Human Ortholog: 12p12.1 Cellular Component: cytosol; membrane; nucleoplasm; nucleus Molecular Function:N-acylneuraminate cytidylyltransferase activity
NCBI Summary:	This gene encodes an enzyme that converts N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc). This process is important in the formation of sialylated glycoprotein and glycolipids. This modification plays a role in cell-cell communications and immune responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Code:	Q8NFW8
NCBI GenInfo Identifier:	68059539
NCBI Gene ID:	55907
NCBI Accession:	Q8NFW8.2
UniProt Secondary Accession:	Q8NFW8,Q96AX5, Q9NQZ0,
UniProt Related Accession:	Q8NFW8
Molecular Weight:	29,561 Da
NCBI Full Name:	N-acylneuraminate cytidylyltransferase
NCBI Synonym Full Names:	cytidine monophosphate N-acetylneuraminic acid synthetase
NCBI Official Symbol:	CMAS
NCBI Official Synonym Symbols:	CSS
NCBI Protein Information:	N-acylneuraminate cytidylyltransferase
UniProt Protein Name:	N-acylneuraminate cytidylyltransferase
UniProt Synonym Protein Names:	CMP-N-acetylneuraminic acid synthase; CMP-NeuNAc synthase
Protein Family:	N-acylneuraminate cytidylyltransferase
UniProt Gene Name:	CMAS
UniProt Entry Name:	NEUA_HUMAN

	Western blot analysis of extracts of various cell lines, using CMAS Antibody (CAB13752) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat brain using CMAS antibody (CAB13752) at dilution of 1:150 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using CMAS antibody (CAB13752) at dilution of 1:150 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)