Anti-CES2 Antibody (CAB1514)

Antibody Name:	Anti-CES2 Antibody
Antibody SKU:	CAB1514
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 310-410 of human CES2 (NP_003860.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, Mouse liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 310-410 of human CES2 (NP_003860.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IPGV VDGV FLPR HPQE LLAS ADFQ PVPS IVGV NNNE FGWL IPKV MRIY DTQK EMDR EASQ AALQ KMLT LLML PPTF GDLL REEY IGDN GDPQ TLQA QFQE M
Gene ID:	8824
Uniprot:	O00748
Cellular Location:	Endoplasmic reticulum lumen
Calculated MW:	59kDa/61kDa
Observed MW:	67kDa

Synonyms:

CES2, CE-2, CES2A1, PCE-2, iCE

Background:

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Endoplasmic reticulum; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 3.1.1.84; EC 3.1.1.1; EC 3.1.1.56; Hydrolase Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: extracellular space; endoplasmic reticulum lumen; endoplasmic reticulum Molecular Function:methylumbelliferyl-acetate deacetylase activity Biological Process: catabolic process
NCBI Summary:	This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2010]
UniProt Code:	O00748
NCBI GenInfo Identifier:	46576349
NCBI Gene ID:	8824
NCBI Accession:	O00748.1
UniProt Secondary Accession:	O00748,Q16859, Q5MAB8, Q7Z366, Q8IUP4, Q8TCP8, A8K367
UniProt Related Accession:	O00748
Molecular Weight:	623
NCBI Full Name:	Cocaine esterase
NCBI Synonym Full Names:	carboxylesterase 2
NCBI Official Symbol:	CES2
NCBI Official Synonym Symbols:	iCE; CE-2; PCE-2; CES2A1
NCBI Protein Information:	cocaine esterase; hCE-2; carboxylesterase 2 (intestine, liver); methylumbelliferyl-acetate deacetylase 2; intestinal carboxylesterase; liver carboxylesterase-2
UniProt Protein Name:	Cocaine esterase
UniProt Synonym Protein Names:	Carboxylesterase 2 (EC:3.1.1.1); CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2 (EC:3.1.1.56)
Protein Family:	Liver carboxylesterase
UniProt Gene Name:	CES2
UniProt Entry Name:	EST2_HUMAN

	Western blot analysis of extracts of various cell lines, using CES2 antibody (CAB1514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human colon using CES2 antibody (CAB1514) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using CES2 antibody (CAB1514). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-CES2 Antibody (CAB13640)	Human CES2 / Cocaine esterase ELISA Kit
CES2 Antibody (PACO05627)	Human Cocaine esterase (CES2) ELISA Kit
CES2 Antibody (PACO08374)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)