Description
COL4A4 Polyclonal Antibody (CAB22870)
The COL4A4 Polyclonal Antibody (CAB22870) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human COL4A4 and has been rigorously validated for use in Western blot applications.COL4A4 is vital for kidney function and mutations in the COL4A4 gene have been linked to kidney diseases, including Alport syndrome. By targeting COL4A4, researchers can investigate the molecular mechanisms underlying these diseases and explore potential therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | COL4A4 Polyclonal Antibody |
| SKU: | CAB22870 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | BFH, ATS2, BFH1, CA44, COL4A4 |
| Positive Sample: | 293F |
| Calculated MW: | 164kDa |
| Observed MW: | 160kDa |
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR.
| Purification Method | Affinity purification |
| Gene ID | 1286 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from 293F cells, using COL4A4 Rabbit pAb (CAB22870) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s. |
