The COMMD5 Antibody (CAB16518) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to be involved in proximal tubule morphogenesis. Located in cytosol and nucleoplasm.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
COMMD5 Antibody
SKU:
CAB16518
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCARG, HT002, COMMD5
Positive Sample:
MCF7, PC-3, 293F
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
25kDa
Observed MW:
30kDa
Predicted to be involved in proximal tubule morphogenesis. Located in cytosol and nucleoplasm.
Purification Method
Affinity purification
Gene ID
28991
RRID
AB_2769004
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using COMMD5 Rabbit pAb (CAB16518) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of C6 cells using COMMD5 Rabbit pAb (CAB16518) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
