The COMT Antibody (CAB6200) is a high-quality antibody developed for reliable detection and analysis of target proteins. Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. RRID AB_2766810 Gene ID 1312 Swiss Prot Synonym HEL-S-98n; COMT
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
COMT Antibody
SKU:
CAB6200
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HEL-S-98n, COMT
Positive Sample:
MCF7, HT-29, THP-1, A-431, HepG2, Mouse liver, Rat liver
Cellular Localization:
Cell Membrane, Cytoplasm, Extracellular Side, Single-Pass Type Ii Membrane Protein.
Calculated MW:
30kDa
Observed MW:
28-32kDa
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. RRID AB_2766810 Gene ID 1312 Swiss Prot Synonym HEL-S-98n; COMT
Purification Method:
Affinity purification
Gene ID:
1312
RRID:
AB_2766810
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using COMT Rabbit pAb (CAB6200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates using COMT Rabbit pAb (CAB6200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
![[KD Validated] COMT Polyclonal Antibody (CAB23600)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/223932/583857/kd-validated-comt-polyclonal-antibody__55967.1701185411.jpg?c=2 "[KD Validated] COMT Polyclonal Antibody (CAB23600)")
