Anti-COPS5 Antibody (CAB1766)

Antibody Name:	Anti-COPS5 Antibody
Antibody SKU:	CAB1766
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human COPS5 (NP_006828.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U2OS, HeLa, MCF7, NIH/3T3, K-562, U-251MG, Mouse brain, Mouse heart, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-334 of human COPS5 (NP_006828.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAS GSGM AQKT WELA NNMQ EAQS IDEI YKYD KKQQ QEIL AAKP WTKD HHYF KYCK ISAL ALLK MVMH ARSG GNLE VMGL MLGK VDGE TMII MDSF ALPV EGTE TRVN AQAA AYEY MAAY IENA KQVG RLEN AIGW YHSH PGYG CWLS GIDV STQM LNQQ FQEP FVAV VIDP TRTI SAGK VNLG AFRT YPKG YKPP DEGP SEYQ TIPL NKIE DFGV HCKQ YYAL EVSY FKSS LDRK LLEL LWNK YWVN TLSS SSLL TNAD YTTG QVFD LSEK LEQS EAQL GRGS FMLG LETH DRKS EDKL AKAT RDSC KTTI EAIH GLMS QVIK DKLF NQIN IS
Gene ID:	10987
Uniprot:	Q92905
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Nucleus, perinuclear region, secretory vesicle, synaptic vesicle
Calculated MW:	37kDa
Observed MW:	37KDa

Synonyms:

COPS5, CSN5, JAB1, MOV-34, SGN5

Background:

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors.

UniProt Protein Function:	COPS5: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS1, COPS2, COPS4, COPS6 and COPS7 (COPS7A or COPS7B). The CSN complex interacts with the BRISC complex. Also exists as monomeric form. Interacts with TP53, MIF, JUN, UCHL1, NCOA1, HIF1A, p27Kip1, BCL3, GFER, PGR, LHCGR, SMAD4, SMAD7, ID1, ID3, ITGB2 and TOP2A. Part of a complex consisting of RANBP9, Ran, DYRK1B and COPS5. Belongs to the peptidase M67A family. CSN5 subfamily.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Translation; Protease; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; EC 3.4.-.- Chromosomal Location of Human Ortholog: 8q13.1 Cellular Component: cytoplasm; eukaryotic translation initiation factor 3 complex; nucleoplasm; nucleus; signalosome; synaptic vesicle Molecular Function:metallopeptidase activity; protein binding; transcription coactivator activity; translation initiation factor activity; ubiquitin-specific protease activity Biological Process: cullin deneddylation; nucleotide-excision repair, DNA damage recognition; positive regulation of transcription from RNA polymerase II promoter; protein deneddylation; protein deubiquitination; regulation of JNK cascade; transcription from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; translation
NCBI Summary:	The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92905
NCBI GenInfo Identifier:	55976562
NCBI Gene ID:	10987
NCBI Accession:	Q92905.4
UniProt Secondary Accession:	Q92905,O15386, Q6AW95, Q86WQ4, Q9BQ17,
UniProt Related Accession:	Q92905
Molecular Weight:	37,579 Da
NCBI Full Name:	COP9 signalosome complex subunit 5
NCBI Synonym Full Names:	COP9 signalosome subunit 5
NCBI Official Symbol:	COPS5
NCBI Official Synonym Symbols:	CSN5; JAB1; SGN5; MOV-34
NCBI Protein Information:	COP9 signalosome complex subunit 5
UniProt Protein Name:	COP9 signalosome complex subunit 5
UniProt Synonym Protein Names:	Jun activation domain-binding protein 1
UniProt Gene Name:	COPS5
UniProt Entry Name:	CSN5_HUMAN

	Western blot analysis of extracts of various cell lines, using COPS5 Rabbit pAb (CAB1766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using COPS5 antibody (CAB1766) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver using COPS5 antibody (CAB1766) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-COPS5 Antibody (CAB13401)	HumanÂ COPS5 (COP9Â signalosomeÂ complexÂ subunitÂ 5Â )Â ELISAÂ Kit (HUFI03389)
Anti-JAB1 Antibody (CAB4087)	COPS5 Colorimetric Cell-Based ELISA
COPS5 Antibody (PACO06593)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)