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Anti-COPS7A Antibody (CAB8212)

SKU:
CAB8212
Product Type:
Antibody
Applications:
WB
IF
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 3 Anti-COPS7A Antibody CAB8212
  • Immunology Antibodies 3 Anti-COPS7A Antibody CAB8212
€139 - €419
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Description

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Antibody Name:COPS7A Rabbit Polyclonal Antibody
Antibody SKU:CAB8212
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human COPS7A (NP_057403.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, NCI-H460, A-549, Mouse brain, Mouse heart, Mouse kidney, Rat heart, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human COPS7A (NP_057403.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSAE VKVT GQNQ EQFL LLAK SAKG AALA TLIH QVLE APGV YVFG ELLD MPNV RELA ESDF ASTF RLLT VFAY GTYA DYLA EARN LPPL TEAQ KNKL RHLS VVTL AAKV KCIP YAVL LEAL ALRN VRQL EDLV IEAV YADV LRGS LDQR NQRL EVDY SIGR DIQR QDLS AIAR TLQE WCVG CEVV LSGI EEQV SRAN QHKE QQLG LKQQ IESE VANL KKTI KVTT AAAA AATS QDPE QHLT ELRE PAPG TNQR QPSK KASK GKGL RGSA KIWS KSN
Gene ID:50813
Uniprot:Q9UBW8
Cellular Location:Cytoplasm, Nucleus
Calculated MW:30kDa
Observed MW:30kDa
Synonyms:COPS7A, CSN7, CSN7A, SGN7a
Background:This gene encodes a component of the COP9 signalosome, an evolutionarily conserved multi-subunit protease that regulates the activity of the ubiquitin conjugation pathway. Alternatively spliced transcript variants that encode the same protein have been described.
UniProt Protein Function:COPS7A: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Belongs to the CSN7/EIF3M family. CSN7 subfamily.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytoplasm; nucleoplasm; signalosome

Molecular Function:protein binding

Biological Process: cullin deneddylation; nucleotide-excision repair, DNA damage recognition; transcription-coupled nucleotide-excision repair

NCBI Summary:This gene encodes a component of the COP9 signalosome, an evolutionarily conserved multi-subunit protease that regulates the activity of the ubiquitin conjugation pathway. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Mar 2014]
UniProt Code:Q9UBW8
NCBI GenInfo Identifier:55976618
NCBI Gene ID:50813
NCBI Accession:Q9UBW8.1
UniProt Secondary Accession:Q9UBW8,Q9NVX3, Q9UJW4, A8K9A6,
UniProt Related Accession:Q9UBW8
Molecular Weight:30,277 Da
NCBI Full Name:COP9 signalosome complex subunit 7a
NCBI Synonym Full Names:COP9 signalosome subunit 7A
NCBI Official Symbol:COPS7A  
NCBI Official Synonym Symbols:CSN7; CSN7A; SGN7a  
NCBI Protein Information:COP9 signalosome complex subunit 7a
UniProt Protein Name:COP9 signalosome complex subunit 7a
UniProt Synonym Protein Names:Dermal papilla-derived protein 10; JAB1-containing signalosome subunit 7a
UniProt Gene Name:COPS7A  
UniProt Entry Name:CSN7A_HUMAN
Anti-COPS7A Polyclonal Antibody (CAB8212)Western blot analysis of extracts of various cell lines, using COPS7A antibody (CAB8212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-COPS7A Polyclonal Antibody (CAB8212)Immunofluorescence analysis of U2OS cells using COPS7A antibody (CAB8212) at dilution of 1:100. Blue: DAPI for nuclear staining.

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