The COVID-19 Neutralizing Antibody ELISA Kit is a cutting-edge tool for the precise measurement of neutralizing antibodies against the COVID-19 virus in human serum and plasma samples. This innovative kit offers high sensitivity and specificity, allowing for accurate and reliable results in various research settings. Neutralizing antibodies play a key role in the immune response to viral infections, including COVID-19. By detecting and quantifying these antibodies, researchers can gain valuable insights into immune protection against the virus, track immune responses over time, and assess the effectiveness of vaccines and treatments.
The COVID-19 Neutralizing Antibody ELISA Kit is a valuable resource for studying the immune response to COVID-19, evaluating vaccine efficacy, and identifying potential therapeutic strategies. With its user-friendly design and robust performance, this kit is an essential tool for advancing research efforts in the fight against the COVID-19 pandemic.
Product Name:
COVID-19 Neutralizing Antibody ELISA Kit
SKU:
AEFI01014
Reactivity:
Universal
Assay Type:
Sandwich ELISA, Double Antigen
Sensitivity:
0.938 ng/mL
Range:
1.563-100 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-97%
86-98%
80-93%
EDTA Plasma (n = 5)
87-105%
85-100%
81-97%
Heparin Plasma (n = 5)
85-103%
86-100%
81-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-104
94
EDTA Plasma (n = 5)
86-105
99
Heparin Plasma (n = 5)
86-105
92
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Biotin-Labeled Antigen Binding: Add biotin-labeled antigen working solution and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add SABC working solution and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes until color develops.
6
Stop Reaction & Reading: Add stop solution and read absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes and collect supernatant.
Plasma
Collect in anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue with PBS or lysis buffer, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells on ice using lysis buffer, centrifuge and collect supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antigen (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
