The COVID-19 Spike Protein S1 ELISA Kit is a cutting-edge assay designed for the precise measurement of the S1 subunit of the spike protein of the novel coronavirus in various biological samples. This kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a multitude of research purposes. The spike protein S1 subunit is a key component of the COVID-19 virus and is essential for viral entry into host cells.
By detecting and measuring levels of the spike protein S1 subunit, researchers can gain valuable insights into viral infection and pathogenesis, as well as evaluate the efficacy of vaccines and antiviral therapies. Overall, the COVID-19 Spike Protein S1 ELISA Kit is an indispensable tool for the study of COVID-19 and the development of strategies to combat the ongoing global pandemic.
Product Name:
COVID-19 Spike protein (S1) ELISA Kit
SKU:
AEFI01004
Reactivity:
Coronavirus (COVID-19)
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.938 pg/mL
Range:
1.563-100 pg/mL
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
95-105%
98-104%
89-100%
EDTA Plasma (n = 5)
82-95%
85-94%
82-96%
Heparin Plasma (n = 5)
91-98%
84-100%
86-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
92-104
98
EDTA Plasma (n = 5)
85-101
95
Heparin Plasma (n = 5)
87-105
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
