Anti-COX6A1 Antibody (CAB3798)

Antibody Name:	Anti-COX6A1 Antibody
Antibody SKU:	CAB3798
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1 (NP_004364.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:20 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, MCF7, Mouse kidney, Mouse heart, Mouse liver, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1 (NP_004364.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSGA HGEE GSAR MWKT LTFF VALP GVAV SMLN VYLK SHHG EHER PEFI AYPH LRIR TKPF PWGD GNHT LFHN PHVN PLPT GYED E
Gene ID:	1337
Uniprot:	P12074
Cellular Location:	Mitochondrion inner membrane
Calculated MW:	12kDa
Observed MW:	12kDa

Synonyms:

COX6A1, CMTRID, COX6A, COX6AL

Background:

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented.

UniProt Protein Function:	COX6A1: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 6A family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12q24.2\|12q24.2 Cellular Component: mitochondrial inner membrane Biological Process: generation of precursor metabolites and energy; mitochondrial electron transport, cytochrome c to oxygen Disease: Charcot-marie-tooth Disease, Recessive Intermediate D
NCBI Summary:	Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented. [provided by RefSeq, Jul 2008]
UniProt Code:	P12074
NCBI GenInfo Identifier:	6166030
NCBI Gene ID:	1337
NCBI Accession:	P12074.4
UniProt Secondary Accession:	P12074,O43714, Q32Q37, B2R500,
UniProt Related Accession:	P12074
Molecular Weight:	12,155 Da
NCBI Full Name:	Cytochrome c oxidase subunit 6A1, mitochondrial
NCBI Synonym Full Names:	cytochrome c oxidase subunit 6A1
NCBI Official Symbol:	COX6A1
NCBI Official Synonym Symbols:	COX6A; CMTRID; COX6AL
NCBI Protein Information:	cytochrome c oxidase subunit 6A1, mitochondrial
UniProt Protein Name:	Cytochrome c oxidase subunit 6A1, mitochondrial
UniProt Synonym Protein Names:	Cytochrome c oxidase polypeptide VIa-liver; Cytochrome c oxidase subunit VIA-liver; COX VIa-L
Protein Family:	Cytochrome c oxidase
UniProt Gene Name:	COX6A1
UniProt Entry Name:	CX6A1_HUMAN

	Western blot analysis of extracts of various cell lines, using COX6A1 antibody (CAB3798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat cerebellum using COX6A1 antibody (CAB3798) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using COX6A1 antibody (CAB3798) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using COX6A1 antibody (CAB3798) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using COX6A1 Polyclonal Antibody (CAB3798) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

COX6A1 Antibody (PACO08549)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)