The COX6C Antibody (CAB16250) is a high-quality antibody developed for reliable detection and analysis of target proteins. Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIc, which has 77% amino acid sequence identity with mouse subunit VIc. This gene is up-regulated in prostate cancer cells. A pseudogene has been found on chromosomes 16p12. RRID AB_2769017 Gene ID 1345 Swiss Prot Synonym COX6C
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
COX6C Antibody
SKU:
CAB16250
Size:
100μL, 20μL
Reactivity:
Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
COX6C
Positive Sample:
-
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrial Respiratory Chain Complex Iv, Mitochondrion.
Calculated MW:
9kDa
Observed MW:
Refer to figures
Cytochrome c oxidase, the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIc, which has 77% amino acid sequence identity with mouse subunit VIc. This gene is up-regulated in prostate cancer cells. A pseudogene has been found on chromosomes 16p12. RRID AB_2769017 Gene ID 1345 Swiss Prot Synonym COX6C
Purification Method:
Affinity purification
Gene ID:
1345
RRID:
AB_2769017
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunofluorescence analysis of NIH/3T3 cells using COX6C Rabbit pAb (CAB16250) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
