Anti-CPA6 Antibody (CAB13644)

Antibody Name:	Anti-CPA6 Antibody
Antibody SKU:	CAB13644
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-437 of human CPA6 (NP_065094.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, HepG2, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 130-437 of human CPA6 (NP_065094.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLSG YNYE VYHS LEEI QNWM HHLN KTHS GLIH MFSI GRSY EGRS LFIL KLGR RSRL KRAV WIDC GIHA REWI GPAF CQWF VKEA LLTY KSDP AMRK MLNH LYFY IMPV FNVD GYHF SWTN DRFW RKTR SRNS RFRC RGVD ANRN WKVK WCDE GASM HPCD DTYC GPFP ESEP EVKA VANF LRKH RKHI RAYL SFHA YAQM LLYP YSYK YATI PNFR CVES AAYK AVNA LQSV YGVR YRYG PAST TLYV SSGS SMDW AYKN GIPY AFAF ELRD TGYF GFLL PEML IKPT CTET MLAV KNIT MHLL KKCP
Gene ID:	57094
Uniprot:	Q8N4T0
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	10kDa/37kDa/51kDa
Observed MW:	51kDa

Synonyms:

CPA6, CPAH, ETL5, FEB11

Background:

The gene encodes a member of the peptidase M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature enzyme, which catalyzes the release of large hydrophobic C-terminal amino acids. This enzyme has functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Mutations in this gene may be linked to epilepsy and febrile seizures, and a translocation t(6;8)(q26;q13) involving this gene has been associated with Duane retraction syndrome.

UniProt Protein Function:	CPA6: May be involved in the proteolytic inactivation of enkephalins and neurotensin in some brain areas. May convert inactive angiotensin I into the biologically active angiotensin II. A chromosomal aberration involving CPA6 was found in a patient with Duane retraction syndrome. Translocation t(6;8)(q26;q13). Defects in CPA6 are the cause of epilepsy, familial temporal lobe, type 5 (ETL5). ETL5 is a focal form of epilepsy characterized by recurrent seizures that arise from foci within the temporal lobe. Seizures are usually accompanied by sensory symptoms, most often auditory in nature. Defects in CPA6 are the cause of familial febrile convulsions type 11 (FEB11). FEB11 consists of seizures associated with febrile episodes in childhood without any evidence of intracranial infection or defined pathologic or traumatic cause. It is a common condition, affecting 2-5% of children aged 3 months to 5 years. The majority are simple febrile seizures (generally defined as generalized onset, single seizures with a duration of less than 30 minutes). Complex febrile seizures are characterized by focal onset, duration greater than 30 minutes, and/or more than one seizure in a 24 hour period. The likelihood of developing epilepsy following simple febrile seizures is low. Complex febrile seizures are associated with a moderately increased incidence of epilepsy. Belongs to the peptidase M14 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; EC 3.4.17.1; Secreted; Protease Chromosomal Location of Human Ortholog: 8q13.2 Cellular Component: proteinaceous extracellular matrix Molecular Function:zinc ion binding; metallocarboxypeptidase activity Biological Process: proteolysis Disease: Epilepsy, Familial Temporal Lobe, 5; Febrile Seizures, Familial, 11
NCBI Summary:	The gene encodes a member of the peptidase M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature enzyme, which catalyzes the release of large hydrophobic C-terminal amino acids. This enzyme has functions ranging from digestion of food to selective biosynthesis of neuroendocrine peptides. Mutations in this gene may be linked to epilepsy and febrile seizures, and a translocation t(6;8)(q26;q13) involving this gene has been associated with Duane retraction syndrome. [provided by RefSeq, May 2016]
UniProt Code:	Q8N4T0
NCBI GenInfo Identifier:	85683250
NCBI Gene ID:	57094
NCBI Accession:	Q8N4T0.2
UniProt Related Accession:	Q8N4T0
Molecular Weight:	10kDa; 37kDa; 51kDa
NCBI Full Name:	Carboxypeptidase A6
NCBI Synonym Full Names:	carboxypeptidase A6
NCBI Official Symbol:	CPA6
NCBI Official Synonym Symbols:	CPAH; ETL5; FEB11
NCBI Protein Information:	carboxypeptidase A6
UniProt Protein Name:	Carboxypeptidase A6
Protein Family:	Carboxypeptidase
UniProt Gene Name:	CPA6
UniProt Entry Name:	CBPA6_HUMAN

	Western blot analysis of extracts of various cell lines, using CPA6 antibody (CAB13644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using CPA6 antibody (CAB13644) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver using CPA6 antibody (CAB13644) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using CPA6 antibody (CAB13644) at dilution of 1:100 (40x lens).

Antibodies

Anti-CPA6 Antibody (CAB6475)

CPA6 Antibody (PACO08562)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)