The CPOX Antibody (CAB3807) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for a variety of applications such as Western blotting and immunohistochemistry.CPOX is essential for the production of heme, a molecule involved in various biological processes including oxygen transport, energy production, and drug metabolism. Dysregulation of CPOX activity has been linked to diseases such as porphyria, a group of metabolic disorders characterized by the accumulation of heme precursors.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CPOX Antibody
SKU:
CAB3807
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
COX, CPO, CPX, HCP, HARPO, CPOX
Positive Sample:
HeLa, A375, HepG2, A-549, Mouse liver, Rat liver
Cellular Localization:
Mitochondrion Intermembrane Space.
Calculated MW:
50kDa
Observed MW:
36kDa
The protein encoded by this gene is the sixth enzyme of the heme biosynthetic pathway. The encoded enzyme is soluble and found in the intermembrane space of mitochondria. This enzyme catalyzes the stepwise oxidative decarboxylation of coproporphyrinogen III to protoporphyrinogen IX, a precursor of heme. Defects in this gene are a cause of hereditary coproporphyria (HCP).
Purification Method
Affinity purification
Gene ID
1371
RRID
AB_2765315
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CPOX Rabbit pAb (CAB3807) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
