Anti-CPT1B Antibody (CAB6796)

Antibody Name:	Anti-CPT1B Antibody
Antibody SKU:	CAB6796
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, B cells, Mouse kidney, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SYQV AKAL ADDV ELYC FQFL PFGK GLIK KCRT SPDA FVQI ALQL AHFR DRGK FCLT YEAS MTRM FREG RTET VRSC TSES TAFV QAMM EGSH TKAD LRDL FQKA AKKH QNMY RLAM TGAG IDRH LFCL YLVS KYLG VSSP FLAE VLSE PWRL STSQ IPQS QIRM FDPE QHPN HLGA GGGF GPVA DDGY GVSY MIAG ENTI FFHI SSKF SSSE TNAQ RFGN HIRK ALLD IADL FQVP KAYS
Gene ID:	1375
Uniprot:	Q92523
Cellular Location:	Mitochondrion outer membrane, Multi-pass membrane protein
Calculated MW:	66kDa/78kDa/83kDa/87kDa
Observed MW:	110kDa

Synonyms:

CPT1B, CPT1-M, CPT1M, CPTI, CPTI-M, M-CPT1, MCCPT1, MCPT1

Background:

The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.

UniProt Protein Function:	CPT1B: a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]
UniProt Protein Details:	Protein type:Transferase; Lipid Metabolism - fatty acid; Membrane protein, multi-pass; EC 2.3.1.21; Membrane protein, integral Chromosomal Location of Human Ortholog: 22q13.33 Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane Molecular Function:carnitine O-palmitoyltransferase activity Biological Process: fatty acid beta-oxidation; carnitine shuttle; cellular lipid metabolic process
NCBI Summary:	The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]
UniProt Code:	Q92523
NCBI GenInfo Identifier:	2493497
NCBI Gene ID:	1375
NCBI Accession:	Q92523.2
UniProt Secondary Accession:	Q92523,Q13389, Q99655, Q9BY90, B7Z4U4, B7Z5T8, E9PCP2
UniProt Related Accession:	Q92523
Molecular Weight:	87,801 Da
NCBI Full Name:	Carnitine O-palmitoyltransferase 1, muscle isoform
NCBI Synonym Full Names:	carnitine palmitoyltransferase 1B (muscle)
NCBI Official Symbol:	CPT1B
NCBI Official Synonym Symbols:	CPTI; CPT1M; MCPT1; CPT1-M; CPTI-M; M-CPT1; MCCPT1
NCBI Protein Information:	carnitine O-palmitoyltransferase 1, muscle isoform; carnitine palmitoyltransferase I-like protein; carnitine O-palmitoyltransferase I, mitochondrial muscle isoform
UniProt Protein Name:	Carnitine O-palmitoyltransferase 1, muscle isoform
UniProt Synonym Protein Names:	Carnitine O-palmitoyltransferase I, muscle isoform; CPT I; CPTI-M; Carnitine palmitoyltransferase 1B; Carnitine palmitoyltransferase I-like protein
Protein Family:	Carnitine O-palmitoyltransferase
UniProt Gene Name:	CPT1B
UniProt Entry Name:	CPT1B_HUMAN

	Western blot analysis of extracts of various cell lines, using CPT1B antibody (CAB6796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat liver using CPT1B antibody (CAB6796) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using CPT1B antibody (CAB6796) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using CPT1B antibody (CAB6796) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH/3T3 cells using CPT1B antibody (CAB6796) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

CPT1B Antibody (PACO06940)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)