Anti-CREM Antibody (CAB5624)

Antibody Name:	Anti-CREM Antibody
Antibody SKU:	CAB5624
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CREM (NP_853549.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, K-562, Mouse testis, Rat testis

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CREM (NP_853549.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKC ARKK YIKT NPRQ MTME TVES QHDG SITA SLTE SKSA HVQT QTGQ NSIP ALAQ VAAI AETD ESAE SEGV IDSH KRRE ILSR RPSY RKIL NELS SDVP
Gene ID:	1390
Uniprot:	Q03060
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	11-38kDa
Observed MW:	39kDa

Synonyms:

CREM, CREM-2, ICER, hCREM-2

Background:

This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.

UniProt Protein Function:	CREM: a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. An important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative splice isoforms allow this protein to exert spatial and temporal specificity to cAMP responsiveness. Twentyeight alternatively spliced isoforms of the human protein have been described, with some of them functioning as activators and some as repressors of transcription.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 10p11.21 Cellular Component: transcription factor complex; cytoplasm; nucleus Molecular Function:protein binding; DNA binding; cAMP response element binding protein binding; transcription factor activity Biological Process: response to cAMP; transcription, DNA-dependent; regulation of transcription, DNA-dependent; multicellular organismal development; spermatogenesis; glycosphingolipid metabolic process; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; signal transduction; cell differentiation; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq, Jul 2008]
UniProt Code:	Q03060
NCBI GenInfo Identifier:	259016392
NCBI Gene ID:	1390
NCBI Accession:	Q03060.5
UniProt Secondary Accession:	Q03060,O75519, Q14501, A8K014, A8K3J7, A8K6A1, A8MPQ2 B4DXC1, C9J785, C9JZ10, E9PAR4, E9PHM1,
UniProt Related Accession:	Q03060
Molecular Weight:	361
NCBI Full Name:	cAMP-responsive element modulator
NCBI Synonym Full Names:	cAMP responsive element modulator
NCBI Official Symbol:	CREM
NCBI Official Synonym Symbols:	ICER; CREM-2; hCREM-2
NCBI Protein Information:	cAMP-responsive element modulator; CREM 2beta-a protein; CREM 2alpha-b protein; cAMP response element modulator; inducible cAMP early repressor ICER
UniProt Protein Name:	cAMP-responsive element modulator
UniProt Synonym Protein Names:	Inducible cAMP early repressor; ICER
Protein Family:	cAMP-responsive element modulator
UniProt Gene Name:	CREM
UniProt Entry Name:	CREM_HUMAN

	Western blot analysis of extracts of various cell lines, using CREM antibody (CAB5624) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using CREM antibody (CAB5624) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using CREM antibody (CAB5624) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
CREM Antibody (PACO03499)	CREM Colorimetric Cell-Based ELISA
CREM Antibody (PACO08598)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)