The CREM Antibody (CAB5624) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-raised antibody is specifically designed for binding to CREM proteins in human samples, making it an ideal choice for Western blot applications.CREM plays a crucial role in modulating gene expression in response to cellular stress, inflammation, and immune signaling pathways. By targeting CREM with this antibody, researchers can easily detect and analyze CREM levels in different cell types, providing valuable insights into the role of CREM in immune regulation and disease processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CREM Antibody
SKU:
CAB5624
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ICER, CREM-2, hCREM-2, CREM
Positive Sample:
HeLa, 293T, Mouse testis, RAW264.7
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
37kDa
Observed MW:
39kDa
This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.
Purification Method
Affinity purification
Gene ID
1390
RRID
AB_2766384
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using CREM Rabbit pAb (CAB5624) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of PC-3 cells using CREM Rabbit pAb (CAB5624) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
