Anti-CRHBP Antibody (CAB6568)

Antibody Name:	Anti-CRHBP Antibody
Antibody SKU:	CAB6568
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-322 of human CRHBP (NP_001873.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-322 of human CRHBP (NP_001873.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YLEL REAA DYDP FLLF SANL KREL AGEQ PYRR ALRC LDML SLQG QFTF TADR PQLH CAAF FISE PEEF ITIH YDQV SIDC QGGD FLKV FDGW ILKG EKFP SSQD HPLP SAER YIDF CESG LSRR SIRS SQNV AMIF FRVH EPGN GFTL TIKT DPNL FPCN VISQ TPNG KFTL VVPH QHRN CSFS IIYP VVIK ISDL TLGH VNGL QLKK SSAG CEGI GDFV ELLG GTGL DPSK MTPL ADLC YPFH GPAQ MKVG CDNT VVRM VSSG KHVN RVTF EYRQ LEPY ELEN PNGN SIGE FCLS GL
Gene ID:	1393
Uniprot:	P24387
Cellular Location:	Secreted
Calculated MW:	36kDa
Observed MW:	36kDa

Synonyms:

CRHBP, CRF-BP, CRFBP

Background:

Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy.

UniProt Protein Function:	CRHBP: Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy. Belongs to the CRF-binding protein family.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Inhibitor; Secreted Chromosomal Location of Human Ortholog: 5q13.3 Cellular Component: dendrite; endoplasmic reticulum; extracellular space; Golgi apparatus; intracellular; microtubule; multivesicular body; nerve terminal; nucleus; perikaryon; secretory granule Molecular Function:corticotropin-releasing hormone binding; peptide binding; protein binding Biological Process: behavioral response to ethanol; cellular response to stress; female pregnancy; hormone-mediated signaling; inflammatory response; learning and/or memory; negative regulation of adrenocorticotropic hormone secretion; regulated secretory pathway; regulation of adrenocorticotropin hormone secretion; signal transduction; synaptic transmission, dopaminergic
NCBI Summary:	Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq, Jul 2008]
UniProt Code:	P24387
NCBI GenInfo Identifier:	544099
NCBI Gene ID:	1393
NCBI Accession:	P24387.2
UniProt Secondary Accession:	P24387,Q53F32, Q6FHT5,
UniProt Related Accession:	P24387
Molecular Weight:	36,144 Da
NCBI Full Name:	Corticotropin-releasing factor-binding protein
NCBI Synonym Full Names:	corticotropin releasing hormone binding protein
NCBI Official Symbol:	CRHBP
NCBI Official Synonym Symbols:	CRFBP; CRF-BP
NCBI Protein Information:	corticotropin-releasing factor-binding protein
UniProt Protein Name:	Corticotropin-releasing factor-binding protein
UniProt Synonym Protein Names:	Corticotropin-releasing hormone-binding protein; CRH-BP
Protein Family:	Corticotropin-releasing factor-binding protein
UniProt Gene Name:	CRHBP
UniProt Entry Name:	CRHBP_HUMAN

	Western blot analysis of extracts of various cell lines, using CRHBP antibody (CAB6568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using CRHBP antibody (CAB6568) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using CRHBP antibody (CAB6568) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using CRHBP antibody (CAB6568) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using CRHBP antibody (CAB6568). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-CRHBP Antibody (CAB13027)	Human CRHBP ELISA Kit
Anti-CRHBP Antibody (CAB13028)	Human CRHBP (Corticotropin Releasing Hormone Bingding Protein) CLIA Kit (HUES00869)
	Human Corticotropin-releasing factor-binding protein (CRHBP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)