Anti-CSRP2BP Antibody (CAB7380)

Antibody Name:	Anti-CSRP2BP Antibody
Antibody SKU:	CAB7380
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 523-782 of human CSRP2BP (NP_065397.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HL-60, A375, SW480, Mouse kidney, Mouse heart, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 523-782 of human CSRP2BP (NP_065397.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DGIY GAKE GGIS RLPA GQAT YRTT CQDF RILD RYQT SLPS RKGF RHQT TKFL YRLV GSED MAVD QSIV SPYT SRIL KPYI RRDY ETKP PKLQ LLSQ IRSH LHRS DPHW TPEP DAPL DYCY VRPN HIPT INSM CQEF FWPG IDLS ECLQ YPDF SVVV LYKK VIIA FGFM VPDV KYNE AYIS FLFV HPEW RRAG IATF MIYH LIQT CMGK DVTL HVSA SNPA MLLY QKFG FKTE EYVL DFYD KYYP LEST ECKH AFFL RLRR
Gene ID:	57325
Uniprot:	Q9H8E8
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	74kDa/88kDa
Observed MW:	89kDa

Synonyms:

KAT14, ATAC2, CRP2BP, CSRP2BP, PRO1194, dJ717M23.1

Background:

CSRP2 is a protein containing two LIM domains, which are double zinc finger motifs found in proteins of diverse function. CSRP2 and some related proteins are thought to act as protein adapters, bridging two or more proteins to form a larger protein complex. The protein encoded by this gene binds to one of the LIM domains of CSRP2 and contains an acetyltransferase domain. Although the encoded protein has been detected in the cytoplasm, it is predominantly a nuclear protein. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	CSRP2BP: Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. May function as a scaffold for the ATAC complex to promote ATAC complex stability. Has also weak histone acetyltransferase activity toward histone H4. Required for the normal progression through G1 and G2/M phases of the cell cycle. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Unknown function Chromosomal Location of Human Ortholog: 20p11.23 Cellular Component: cytoplasm; nucleus Molecular Function:histone acetyltransferase activity; LIM domain binding; protein binding Biological Process: establishment and/or maintenance of chromatin architecture; G2/M transition of mitotic cell cycle
NCBI Summary:	CSRP2 is a protein containing two LIM domains, which are double zinc finger motifs found in proteins of diverse function. CSRP2 and some related proteins are thought to act as protein adapters, bridging two or more proteins to form a larger protein complex. The protein encoded by this gene binds to one of the LIM domains of CSRP2 and contains an acetyltransferase domain. Although the encoded protein has been detected in the cytoplasm, it is predominantly a nuclear protein. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2011]
UniProt Code:	Q9H8E8
NCBI GenInfo Identifier:	308153608
NCBI Gene ID:	57325
NCBI Accession:	Q9H8E8.3
UniProt Related Accession:	Q9H8E8
Molecular Weight:	89 kDa
NCBI Full Name:	Cysteine-rich protein 2-binding protein
NCBI Synonym Full Names:	lysine acetyltransferase 14
NCBI Official Symbol:	KAT14
NCBI Official Synonym Symbols:	ATAC2; CRP2BP; CSRP2BP; PRO1194; dJ717M23.1
NCBI Protein Information:	cysteine-rich protein 2-binding protein
UniProt Protein Name:	Cysteine-rich protein 2-binding protein
UniProt Synonym Protein Names:	ADA2A-containing complex subunit 2; ATAC2; CRP2-binding partner; CRP2BP
Protein Family:	Cysteine-rich protein 2-binding protein
UniProt Gene Name:	CSRP2BP
UniProt Entry Name:	CSR2B_HUMAN

	Western blot analysis of extracts of various cell lines, using CSRP2BP antibody (CAB7380) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human kidney using CSRP2BP antibody (CAB7380) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using CSRP2BP antibody (CAB7380). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)