The CSTF2T Antibody (CAB4540) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CSTF2T protein, this antibody enables accurate detection and analysis in various cell types, making it an excellent choice for studies in molecular biology and gene expression research.CSTF2T plays a key role in the formation of the polyadenylation complex and is essential for the efficient cleavage and polyadenylation of mRNA transcripts. Dysregulation of CSTF2T has been linked to various diseases, including cancer and neurological disorders, highlighting its importance in cellular processes.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CSTF2T Antibody
SKU:
CAB4540
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CstF-64T, CSTF2T
Positive Sample:
Jurkat, U-87MG, K-562, SW620, BT-474, Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
64kDa
Observed MW:
70kDa
Enables RNA binding activity. Predicted to be involved in pre-mRNA cleavage required for polyadenylation. Located in nucleoplasm.
Purification Method
Affinity purification
Gene ID
23283
RRID
AB_2765743
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CSTF2T Rabbit pAb (CAB4540) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunofluorescence analysis of U2OS cells using CSTF2T Rabbit pAb (CAB4540) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
