Western blot analysis of various lysates, using Cathepsin H Rabbit mAb (CAB23573) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Cathepsin H Monoclonal Antibody (CAB23573) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through a monoclonal antibody technology, specifically targets Cathepsin H in human samples and is validated for use in a variety of research applications, including Western blot analysis.Cathepsin H is a lysosomal cysteine protease that has been implicated in various physiological and pathological processes, including immune response modulation and antigen presentation. Its dysregulation has been linked to conditions such as cancer, autoimmune diseases, and inflammatory disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Cathepsin H Monoclonal Antibody
SKU:
CAB23573
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC3082
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACC4, ACC5, CPSB, ACC-4, ACC-5, Cathepsin H
Positive Sample:
A-431, Raji, Mouse lung
Cellular Localization:
Lysosome.
Calculated MW:
37kDa
Observed MW:
28kDa
The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
1512
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Cathepsin H Rabbit mAb (CAB23573) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Mouse lung, using Cathepsin H Rabbit mAb (CAB23573) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
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