The Cullin 1 Monoclonal Antibody (CAB19034) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable ubiquitin protein ligase binding activity and ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex. RRID AB_2862526 Gene ID 8454 Swiss Prot Synonym Cullin 1; CUL1; cullin-1; MGC149834; MGC149835
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Cullin 1 Monoclonal Antibody
SKU:
CAB19034
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0436
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Cullin 1, CUL1, cullin-1, MGC149834, MGC149835
Positive Sample:
A-549, K-562, MCF7, NIH/3T3, Mouse liver, Mouse brain, Rat testis
Cellular Localization:
Cytosol, Nucleoplasm, Plasma Membrane.
Calculated MW:
90kDa
Observed MW:
90kDa
Predicted to enable ubiquitin protein ligase binding activity and ubiquitin-protein transferase activity. Involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process and protein ubiquitination. Located in plasma membrane. Part of Parkin-FBXW7-Cul1 ubiquitin ligase complex and SCF ubiquitin ligase complex. RRID AB_2862526 Gene ID 8454 Swiss Prot Synonym Cullin 1; CUL1; cullin-1; MGC149834; MGC149835
Purification Method:
Affinity purification
Gene ID:
8454
RRID:
AB_2862526
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Cullin 1 Rabbit mAb (CAB19034) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
