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Anti-CUL2 Antibody (CAB5308)

SKU:
CAB5308
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-CUL2 Antibody CAB5308
  • Immunology Antibodies 2 Anti-CUL2 Antibody CAB5308
€139 - €419
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Description

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Antibody Name:Anti-CUL2 Antibody
Antibody SKU:CAB5308
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-310 of human CUL2 (NP_001185708.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human
Positive Samples:PC-3, K-562, HL-60, 293T, OVCAR3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-310 of human CUL2 (NP_001185708.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LEYV ERAT WNDR FSDI YALC VAYP EPLG ERLY TETK IFLE NHVR HLHK RVLE SEEQ VLVM YHRY WEEY SKGA DYMD CLYR YLNT QFIK KNKL TEAD LQYG YGGV DMNE PLME IGEL ALDM WRKL MVEP LQAI LIRM LLRE IKND RGGE DPNQ KVIH GVIN SFVH VEQY KKKF PLKF YQEI FESP FLTE TGEY YKQE ASNL LQES NCSQ YMEK VLGR LKDE EIRC RKYL HPSS YTKV IHEC QQRM VADH LQFL HAEC HNII RQEK KNDM ANM
Gene ID:8453
Uniprot:Q13617
Cellular Location:
Calculated MW:86kDa/89kDa
Observed MW:87kDa
Synonyms:CUL2, cullin-2
Background:
UniProt Protein Function:CUL2: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). Belongs to the cullin family.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: Cul2-RING ubiquitin ligase complex; nucleoplasm; cytosol

Molecular Function:protein binding; ubiquitin protein ligase binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of cell proliferation; viral reproduction; protein ubiquitination; cell cycle arrest; G1/S transition of mitotic cell cycle

UniProt Code:Q13617
NCBI GenInfo Identifier:19863260
NCBI Gene ID:8453
NCBI Accession:Q13617.2
UniProt Secondary Accession:Q13617,O00200, Q5T2B6, Q9UNF9, B3KT95, B7Z6K8, D3DRY6 G3V1S2,
UniProt Related Accession:Q13617
Molecular Weight:745
NCBI Full Name:Cullin-2
NCBI Synonym Full Names:cullin 2
NCBI Official Symbol:CUL2  
NCBI Protein Information:cullin-2; CUL-2
UniProt Protein Name:Cullin-2
Protein Family:Cullin
UniProt Gene Name:CUL2  
UniProt Entry Name:CUL2_HUMAN
Anti-CUL2 Antibody (CAB5308)Western blot analysis of extracts of various cell lines, using CUL2 antibody (CAB5308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CUL2 Antibody (CAB5308)Immunofluorescence analysis of A-549 cells using CUL2 antibody (CAB5308). Blue: DAPI for nuclear staining.

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