Western blot analysis of various lysates, using CYP11A1 Rabbit mAb (CAB23954) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The CYP11A1 Monoclonal Antibody (CAB23954) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using cutting-edge technology, specifically targets CYP11A1 in both human and animal samples, allowing for accurate and reliable detection in various experimental settings.CYP11A1, also known as cytochrome P450scc, is primarily expressed in the adrenal glands and gonads, where it catalyzes the conversion of cholesterol into pregnenolone, the precursor of all steroid hormones. Dysregulation of CYP11A1 activity has been linked to numerous diseases, including adrenal insufficiency, infertility, and hormone-related cancers.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CYP11A1 Monoclonal Antibody
SKU:
CAB23954
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC61692
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CYP11A1, CYP11A, CYPXIA1, P450SCC, cytochrome P450 family 11 subfamily A member 1
Positive Sample:
BeWo, Mouse testis
Cellular Localization:
Mitochondrion Membrane.
Calculated MW:
42kDa/60kDa
Observed MW:
50kDa
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide.
Purification Method
Affinity purification
Gene ID
1583
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CYP11A1 Rabbit mAb (CAB23954) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
