Anti-Aromatase Antibody (CAB12684)

Antibody Name:	Anti-Aromatase Antibody
Antibody SKU:	CAB12684
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Aromatase (NP_000094.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Aromatase (NP_000094.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVLE MLNP IHYN ITSI VPEA MPAA TMPV LLLT GLFL LVWN YEGT SSIP GPGY CMGI GPLI SHGR FLWM GIGS ACNY YNRV YGEF MRVW ISGE ETLI ISKS SSMF HIMK HNHY SSRF GSKL GLQC IGMH EKGI IFNN NPEL WKTT RPFF MKAL SGPG LVRM VTVC AESL KTHL DRLE EVTN
Gene ID:	1588
Uniprot:	P11511
Cellular Location:	Membrane, Peripheral membrane protein
Calculated MW:	24kDa/57kDa
Observed MW:	48kDa

Synonyms:

CYP19A1, ARO, ARO1, CPV1, CYAR, CYP19, CYPXIX, P-450AROM, aromatase, Aromatase

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.

UniProt Protein Function:	CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.
UniProt Protein Details:	Protein type:EC 1.14.14.14; Oxidoreductase; Lipid Metabolism - androgen and estrogen Chromosomal Location of Human Ortholog: 15q21.1 Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; membrane Molecular Function:electron carrier activity; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding; steroid hydroxylase activity Biological Process: estrogen biosynthetic process; steroid biosynthetic process; sterol metabolic process Disease: Aromatase Deficiency; Aromatase Excess Syndrome
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:	P11511
NCBI GenInfo Identifier:	117293
NCBI Gene ID:	1588
NCBI Accession:	P11511.3
UniProt Secondary Accession:	P11511,Q16731, Q3B764, Q58FA0, Q8IYJ7,
UniProt Related Accession:	P11511
Molecular Weight:	24,518 Da
NCBI Full Name:	Aromatase
NCBI Synonym Full Names:	cytochrome P450 family 19 subfamily A member 1
NCBI Official Symbol:	CYP19A1
NCBI Official Synonym Symbols:	ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
NCBI Protein Information:	aromatase
UniProt Protein Name:	Aromatase
UniProt Synonym Protein Names:	CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase
Protein Family:	Aromatase
UniProt Gene Name:	CYP19A1
UniProt Entry Name:	CP19A_HUMAN

	Western blot analysis of extracts of various cell lines, using Aromatase antibody (CAB12684) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded human placenta using Aromatase antibody (CAB12684) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat ovary using Aromatase antibody (CAB12684) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using Aromatase antibody (CAB12684) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-CYP19A1 Antibody (CAB12709)	Human ARO (Aromatase) CLIA Kit (HUES00185)
Anti-Aromatase Antibody (CAB2161)	Human ARO (Aromatase) ELISA Kit (HUES01485)
CYP19A1 Antibody (PACO06396)	Human Cytochrome P450 19A1 (CYP19A1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)