Anti-CYP51A1 Antibody (CAB13485)

Antibody Name:	Anti-CYP51A1 Antibody
Antibody SKU:	CAB13485
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-509 of human CYP51A1 (NP_000777.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 210-509 of human CYP51A1 (NP_000777.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LTAS HCLH GKEI RSQL NEKV AQLY ADLD GGFS HAAW LLPG WLPL PSFR RRDR AHRE IKDI FYKA IQKR RQSQ EKID DILQ TLLD ATYK DGRP LTDD EVAG MLIG LLLA GQHT SSTT SAWM GFFL ARDK TLQK KCYL EQKT VCGE NLPP LTYD QLKD LNLL DRCI KETL RLRP PIMI MMRM ARTP QTVA GYTI PPGH QVCV SPTV NQRL KDSW VERL DFNP DRYL QDNP ASGE KFAY VPFG AGRH RCIG ENFA YVQI KTIW STML RLYE FDLI DGYF PTVN YTTM IHTP ENPV IRYK RRSK
Gene ID:	1595
Uniprot:	Q16850
Cellular Location:	Endoplasmic reticulum membrane, Microsome membrane
Calculated MW:	46kDa/56kDa
Observed MW:

Synonyms:

CYP51A1, CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1, CYP51A1

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CYP51A1: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.14.13.70; Membrane protein, integral; Lipid Metabolism - steroid biosynthesis Chromosomal Location of Human Ortholog: 7q21.2 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane Molecular Function:sterol 14-demethylase activity; iron ion binding; heme binding Biological Process: xenobiotic metabolic process; cholesterol biosynthetic process; steroid biosynthetic process; sterol metabolic process; cholesterol biosynthetic process via 24,25-dihydrolanosterol
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:	Q16850
NCBI GenInfo Identifier:	3915660
NCBI Gene ID:	1595
NCBI Accession:	Q16850.3
UniProt Secondary Accession:	Q16850,O00770, O00772, Q16784, Q8N1A8, Q99868, A4D1F8 B2RAI4, B4DJ55,
UniProt Related Accession:	Q16850
Molecular Weight:	503
NCBI Full Name:	Lanosterol 14-alpha demethylase
NCBI Synonym Full Names:	cytochrome P450, family 51, subfamily A, polypeptide 1
NCBI Official Symbol:	CYP51A1
NCBI Official Synonym Symbols:	LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM
NCBI Protein Information:	lanosterol 14-alpha demethylase; CYPLI; cytochrome P450LI; cytochrome P45014DM; cytochrome P450 51A1; cytochrome P450-14DM; lanosterol 14-alpha-demethylase; cytochrome P450, 51 (lanosterol 14-alpha-demethylase)
UniProt Protein Name:	Lanosterol 14-alpha demethylase
UniProt Synonym Protein Names:	CYPLI; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Sterol 14-alpha demethylase
UniProt Gene Name:	CYP51A1
UniProt Entry Name:	CP51A_HUMAN

	Western blot analysis of extracts of mouse testis, using CYP51A1 antibody (CAB13485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using CYP51A1 antibody (CAB13485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded Mouse kidney using CYP51A1 Rabbit pAb (CAB13485) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat kidney using CYP51A1 Rabbit pAb (CAB13485) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded Human thyroid cancer using CYP51A1 Rabbit pAb (CAB13485) at dilution of 1:50 (40x lens).

Antibodies

Anti-CYP51A1 Antibody (CAB6252)

CYP51A1 Antibody (PACO08726)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)