Anti-CSTA Antibody (CAB5686)

Antibody Name:	Anti-CSTA Antibody
Antibody SKU:	CAB5686
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human CSTA (NP_005204.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human CSTA (NP_005204.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MIPG GLSE AKPA TPEI QEIV DKVK PQLE EKTN ETYG KLEA VQYK TQVV AGTN YYIK VRAG DNKY MHLK VFKS LPGQ NEDL VLTG YQVD KNKD DELT GF
Gene ID:	1475
Uniprot:	P01040
Cellular Location:	Cytoplasm
Calculated MW:	11kDa
Observed MW:	13kDa

Synonyms:

CSTA, AREI, PSS4, STF1, STFA

Background:

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.

UniProt Protein Function:	CSTA: This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis. Defects in CSTA are the cause of ichthyosis exfoliative autosomal recessive ichthyosis bullosa of Siemens-like (AREI). A form of congenital exfoliative ichthyosis, sharing some features with ichthyosis bullosa of Siemens and annular epidermolytic ichthyosis. AREI presents shortly after birth as dry, scaly skin over most of the body with coarse peeling of non- erythematous skin on the palms and soles, which is exacerbated by excessive moisture and minor trauma. Electron microscopy analysis of skin biopsies, reveals mostly normal-appearing upper layers of the epidermis, but prominent intercellular edema of the basal and suprabasal cell layers with aggregates of tonofilaments in the basal keratinocytes. Belongs to the cystatin family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 3q21 Cellular Component: cornified envelope; cytoplasm; extracellular space; nucleus Molecular Function:cysteine protease inhibitor activity; protease binding; protein binding, bridging; structural molecule activity Biological Process: cell-cell adhesion; keratinocyte differentiation; negative regulation of peptidase activity; negative regulation of proteolysis; peptide cross-linking Disease: Exfoliative Ichthyosis, Autosomal Recessive, Ichthyosis Bullosa Of Siemens-like
NCBI Summary:	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer. [provided by RefSeq, Jul 2008]
UniProt Code:	P01040
NCBI GenInfo Identifier:	118177
NCBI Gene ID:	1475
NCBI Accession:	P01040.1
UniProt Secondary Accession:	P01040,Q6IB90,
UniProt Related Accession:	P01040
Molecular Weight:	11,006 Da
NCBI Full Name:	Cystatin-A
NCBI Synonym Full Names:	cystatin A
NCBI Official Symbol:	CSTA
NCBI Official Synonym Symbols:	AREI; PSS4; STF1; STFA
NCBI Protein Information:	cystatin-A
UniProt Protein Name:	Cystatin-A
UniProt Synonym Protein Names:	Cystatin-AS; Stefin-A
Protein Family:	Cystatin
UniProt Gene Name:	CSTA
UniProt Entry Name:	CYTA_HUMAN

	Western blot analysis of extracts of THP-1 cells, using CSTA antibody (CAB5686) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using CSTA antibody (CAB5686). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
CSTA Antibody (PACO06406)	Human Cystatin A ELISA Kit
CSTA Antibody (PACO08646)	Human CSTA (Cystatin A) CLIA Kit (HUES00130)
	Human CSTA (Cystatin A) ELISA Kit (HUES01421)
	Human Cystatin-A (CSTA) ELISA Kit
	Stefin A Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)