The CYTH4 Antibody (CAB14875) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the Cyth4 protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for investigations in cell biology and signal transduction research.Cyth4, also known as Cytohesin 4, plays a crucial role in regulating cellular processes such as cell migration, adhesion, and vesicular trafficking.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
CYTH4 Antibody
SKU:
CAB14875
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CYT4, PSCD4, DJ63G5.1, cytohesin-4, CYTH4
Positive Sample:
Mouse liver, Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Peripheral Membrane Protein.
Calculated MW:
46kDa
Observed MW:
46kDa
This gene encodes a member of the PSCD family of proteins, which have an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family function as GEPs for ADP-ribosylation factors (ARFs), which are guanine nucleotide-binding proteins involved in vesicular trafficking pathways. This protein exhibits GEP activity in vitro with ARF1 and ARF5, but is inactive with ARF6. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
27128
RRID
AB_2761755
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CYTH4 Rabbit pAb (CAB14875) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
